Question

the Lambert-Beer law relates the absorption of light with the concentration and physical properties of a substance. 5 µl of a stock protein solution was diluted to 700 µL with water in a cuvette. the absorbance at 280 nm was 0.24. the extinction coefficient ξ for the protein is 10.3 mM^-1cm^-1.

what is the molar concentration of the original stock protein solution? what is the concentration of the protein solution in µg/µL if the protein molar mass is 35348 Da.

Answer 1

for estimation of protein concentration ii Acc to Larubent Beer Law Absorbane at 1280 x aral whas ilubon Conculcahon of protein Extinchon Extincho coff. &path length protein om factor A280 = 0.24, Extinction coff. = 10. 3mment! path length= lem Mal weight of ritem = 35u8 Da diluhon factors to - 140 putting all the values in equis conc. of - X 35 3u8 X 140 protain 10. 31 88136 mg I me. - 0.082 uglu.