Question

esolving gel with DI water, and blot dry with a paper towel. (demo) a small flask (clean and reuse the old flask), mix com , swirl to mix and add the mixture (with a Pasteur pipet) to the gel ur ponents of the s dly in sert the comb. Some acrylamide may spill out of the gel. Repeat erize at room temperature for 45 min. below should be drawn, completed and entered in PROTOCOLo STACKING GEL-MADE/POURED ON TOP OF RESOLVING GELS VolumeStock reagent and [1 | Final [1? 3.4 mldeionized water 0,83 ml 0.63 ml 0.05 m 0.05 ml 5.0 ul TOTAL: 30% acrylamide mix 1.0 M Tris (pH 6.8) 10%. SDS 10% APS TEMED | 1.0 M Tris ( EED UNTIL PROFESSOR INSTRUCTS YOU TO DO SO. Depending es the same day OR you may store gels until the following week and run structed to run gels the same day, proceed to PROCEDURE 2B, structed to store gels, do the following: gels are fully polymerized, remove them from the plastic clamp and wrap ly in cellophane. Keep the comb in the gel to minimize desiccation. Ke both individually wrapped gels together and wrap both them together. ouble-wrapped in cellophane; individually and collectively. Label a piec me, the date and your lab section and adhere it to the wrapped gels. G They will be stored at 4C until the next lab period.
Not sure how to figure out what formulas to use here

Answer 1

Total volume of the gel = 5 uL
Water = 3.4 mL

0.83 mL of 30% acrylamide mix:
1 mL in 5 mL = 1/5 = 0.2
0.83 mL in 5 mL = 0.83/5 = 0.166
Final Concentration = 0.166*30 = 4.98% =~5%

0.63 mL of 1 M Tris:
0.83 mL in 5 mL = 0.63/5 = 0.126
Final Concentration = 0.126*1 = 0.126 M

0.05 mL of 10% SDS:
0.05 mL in 5 mL = 0.05/5 = 0.01
Final Concentration = 0.01*10 = 0.1%

0.05 mL of 10% APS:
0.05 mL in 5 mL = 0.05/5 = 0.01
Final Concentration = 0.01*10 = 0.1%

5 uL = 0.005 mL of 100% TEMED:
0.005 mL in 5 mL = 0.005/5 = 0.001
Final Concentration = 0.001*10 = 0.01%