The rate of oxygen consumption tells about the rate of electron transport in the mitochondria. Electron transport involves the transport of electrons from electron donors via various carrier complexes to the terminal electron acceptor which is oxygen. During electron transport protons are translocated from matrix to intermembrane space. This proton gradient is used to synthesize ATP from ADP and inorganic phosphate.
In presence of ADP, oxygen consumption would be higher to generate proton gradient which would then aid in conversion of ADP to ATP. The rates would revert to those exisiting before addition of ADP because after a higher oxygen consumption rate enough proton gradient would be produced to convert all of the ADP to ATP. The situation is now similar to that in absence of ADP or prior to addition of ADP .Now mitochondria would return to its basal level of oxygen consumption as before.
Why does the oxygen consumption rate for ADP in the presence of an electron donor revert...
Why does it make physiological sense for the presence of excess ADP to increase the activity of phosphofructokinase in the EMP glycolytic pathway?
How does the addition of ATP affect PFK affinity for ADP?
The figure above is a Lineweaver-Burk plot of P. furiosus PFK with [fructose-6-P] held constant and [ADP] as the variable substrate (dot), in the presence of 5mM ATP (O), or in the presence of 5 mM AMP (X; Tuininga et al., 1999).
- I believed the inhibited protein was ATP synthase, however the
data on Figure 2 does not support this. Please help!
The mitochondria from a patient suffering from muscle pain during physical exercises were analyzed to detect any defect in one of the components of the respiratory chain. Isolated mitochondria were incubated in an isotonic buffer, and changes in the oxygen consumption were measured as various components were sequentially added to the reaction. 1) The following graph (Fig. 1) shows...
Why are two photosystems needed when water is used as an electron donor?
1. The common rate of oxygen consumption of a human is about 15.80 mol per day. The oxygen consumption can be summarized as following reaction: O2 + 4H+ + 4e = 2H2O a. What is current generated by this reaction at the common rate of oxygen consumption? b. Is this current larger or smaller than a current in a computer with power consumption at 8.50 * 102 W at 120.0 V? c. If all the electron flow in a human...
Question 1
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
1) The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a healthy donor (control)....
(A) For the following reaction identify: i the electron donor ii the electron acceptor iii all redox pairs iv reducing agents v oxidizing agent Succinate + FAD+ <--> Fumarate + FADH2 (B) Why could less ATP produced from NADH from glycolysis than NADH from the citric acid cycle? (C) What role does succinate dehydrogenase play in oxidative phosphorylation?
Question 1 a Why in the absence of oxygen does oxaloacetate alone cause an accumulation of citrate? Would any of the other intermediates in the cycle cause an accumulation of citrate in the presence of oxygen? (5 pts) b Toward the end of the paper Krebs states, "While the citric acid cycle thus seems to occur generally in animal tissues, it does not exist in yeast or in E. coli, for yeast and E. coli do not oxidize citric acid...
Which complex in the electron transport chain does not contribute to the proton gradient across the mitochondrial membrane? A. Complex I B. Complex IV C. Complex II D. Complex III Which statement regarding the mitochondrial electron transport chain and oxidative phosphorylation is true? A. Ubiquinone and the F zero subunit of ATP synthase are peripheral membrane proteins. B. Complexes I, II, III, and IV all are proton pumps. C. The final electron acceptor is water. D. Complex II is considered...
Question 1 (25 points).
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
Part 1
The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a...