Based on your knowledge of UV-visible absorption spectroscopy and consideration of the structures of protein chromophores,...
Based on your knowledge of UV-visible absorption spectroscopy and consideration of the structures of protein chromophores, explain why the CD spectrum is more structured in the near-UV than in the far-UV.
Homework Answers
CD or Circular Dichorism spectrum is studied in near UV and far UV for different purposes.
Far-UV CD is usually used to measure protein secondary structure percentages (alpha-helix, beta-sheet, random coil). Near-UV CD looks at conformational changes in a protein by monitoring their effect on the CD spectrum of, predominantly, tryptophan residues.
Protein Chromophore is a moiety that causes a conformational change of molecule on light irradiation.
So CD spectra is more studied in near UV for protein Chromophore study.
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