Question

Based on your knowledge of fluorophores and reading of the spectra of dyes, answer this question. You immunolabel two types of proteins found close to each other like in muscle fiber sarcomeres, but now need to show them together as distinct labeled proteins using fluorescence microscopy. From the list of fluorophores below, choose the best two fluorescent tags to use in combination for fluorescence microscopy

(circle your two choices). Note, there are several examples to choose from to answer this question, but you want the best combination and should consider added issues on use in fluorescence microscopy like those covered in lecture. 4 pts

A. Fluorescein isothiocyanate dye B. Aequorin protein

C. Tetramethylrhodamine dye

D. Acridine Orange dye

E. Alexa 345 dye F.

Alexa 488 dye

G. DAPI dye

*Explain why your chosen two fluorophores work best together in combination to label the specimen.

Describe how colchicine worked to inhibit its target cytoskeleton.

Answer 1