How do you expect a null mutation in the cyclic AMP receptor protein (CRP) would affect...
How do you expect a null mutation in the cyclic AMP receptor protein (CRP) would affect the lac operon in the presence and absence of glucose?
Homework Answers
If null mutation is present a functional CRP can not be synthesized. In the absence of glucose the function of CRP is to activate transcription of operon genes. Now due to null mutation even though glucose is absent the protein will not bind to DNA and transcription of the operon can not be activated. So in the presence of glucose, no transcription of operon because glucose prevents binding of CRP at promoter but due to mutation even though glucose is absent transcription will not occur.
So no transcription of at all in either case
Add Answer to:
How do you expect a null mutation in the cyclic AMP receptor
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The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
1. You are interested in how cyclic-AMP-dependent protein kinase A (PKA) functions to affect learning and...
1. You are interested in how
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brain. It is known that, in the cells you are studying, PKA works
via a signal transduction pathway like the one depicted in Figure
16-15. Furthermore, it is also known that activated PKA
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A. Which strain is the wild-type E.coli? Explain how you know this. B. Which strain contains...
A. Which strain is the wild-type E.coli? Explain how you know
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B. Which strain contains the nonsense mutation in the CRP
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Please can someone help me out with the predictions. Experiment: This “lab" will examine how disruption...
Please can someone help me out with the predictions.
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The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
1. You are interested in how
cyclic-AMP-dependent protein kinase A (PKA) functions to affect
learning and memory, and you decide to study its function in the
brain. It is known that, in the cells you are studying, PKA works
via a signal transduction pathway like the one depicted in Figure
16-15. Furthermore, it is also known that activated PKA
phosphorylates the transcriptional regulator called Nerd that then
activates transcription of the gene Brainy. Which, if any
of the situations described...
A. Which strain is the wild-type E.coli? Explain how you know
this.
B. Which strain contains the nonsense mutation in the CRP
protein gene? Explain how you know this.
C. Which strain contains the deletion mutation in the lac operon
operator sequence? Explain how you know this.
knowledge about the lac operon system AND knowledge Problem set about the Lac operon (these problems require knowledge about the lac operon system AND about consequences of mutations) You are working with three...
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Please can someone help me out with the predictions.
Experiment: This “lab" will examine how disruption (mutation) of parts of a signaling pathway affect the results of activating that pathway. Specifically you will examine the effect of a factor on cell division in normal a cells ( WT- wild type) AND examine the effect of mutations that block the function of: 1. The a factor receptor (STE2 yeast) 2. The cell arrest factor (FAR1 yeast) Wild Type STE mutation FAR...
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