Question

2. As a quality control officer in Company A, you are challenged with sporadic contamination of your pineapple juice product. Initial plating of the juice showed two (2) mixed colony morphologies on general purpose media. With the knowledge that fruit juice products are typically contaminated with yeast and bacteria, describe the methods you will use to isolate and differentiate the microbial contaminants in your pineapple juice.



3. A student has been tasked to observe microorganisms present in pond water under a microscope and draw them. Given that E. coli., amoeba, paramecium, algae, yeast, and Streptococcus are some of the microorganisms present in the pond water, describe the methods the student can employ to accomplish this assignment.


4. As part of microbiology laboratory practical, you are given a mixed culture that contains Salmonella spp., E. coli, Enterococcus spp., Staphylococcus aureus and Saccharomyces cerevisiae and other microorganisms. Describe the methods you can use to isolate and identify Salmonella spp., E. coli and Staphylococcus aureus from the mixed culture?

Answer 1

2.

Pineapple processing plays a crucial role of juice preservation. As the quality of the pineapple juice is impacted by the processing technology applied, the consequences of other preservation techniques and pasteurization on the complete juice quality had been talked about. Throughout juice processing, microorganisms are damaged and chemical changes occur. In order to enhance processing circumstances, awareness of the kinetics of these responses is required, but as of yet, details on the wreckage of the amino acids as well as the change and vitamin C in sugars contents during pineapple juice pasteurization are scanty. Moreover, the kinetics of hydroxymethylfurfural output really should be examined by an accurate method like high-performance fluid chromatography.oxic residues of bacteria in food as well as drink samples may be examined with test kits for bacterial contaminants. Typical test formats for microbial food tests are ELISA assays, agar plates, nutrient plates as well as real time PCR tests.

For detection of pathogenic microorganisms, immunological based techniques (ELISA) can be found. RIDASCREEN® assays are based upon the sandwich engineering, in that the antigen (bacterial area protein) will probably be taken in the well of any microplate and recognized by including a second antibody that is marked with a unique detection enzyme. The immunological detection requires ideal pre-enrichment procedures based on the type of pathogen bacteria.

The detection of bacterial toxins is likely from food samples (RIDASCREEN® SET Total along with RIDASCREEN® SET A,B,C,D,E) or perhaps is utilized for indirect detection of toxin producing pathogenic germs the following to ideal pre enrichment.

The identification of pathogenic germs as well as viruses may be carried out with molecular natural systems, e.g. the extremely precise real time PCR systems of the SureFast® PATHOGEN line. The modular structure involves optimized DNA preparation by spin filtration columns as well as the widely used TaqMan hydrolysis probe know-how associated with FAM dyes for detection. It may be done with all generally available types of real time PCR thermo cyclers. The internal amplification management is integrated in to all PLUS kits. The correct option of amplification management dye depends upon the cycler sort in which the response would be put on to..

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