Question

Materials Para Water M CuSO4 solution Unknown cusO4 solution Simple Dilutions 1 1. Set the spectrophotometer ta wavelength 700 mm and blank against air, 2, Prepare 5.00 ml of the following dilutions of the cuso4 solution using water: 1-2, 1:10, and 1:100.

Answer 1

Aniwer all the questions of D T b? You huve a 05 M stock solution ef Tris base How woudk100 You have a 05 M stock toaftcert(a. (M.unh-1he.tte of the stock 0.5M Use the formula N1 V1 (stock) N2 V2 (working solution) 0.5* V仁0.03.100 V1 30/5-6 Take 6ml of stock solution, and add it to 94ml distilled water to make 100ml of 0.03M working solution. Contentration luw 0388 A . ecl; 0.009 . e ' 2 , 1; e . 0.00912, 10^-6 . 0.0045 ,10A6-4500(M/cm)-1 z. If you perform a 1 4 dlunos on s0mM Tris base at e the ebove standand curve you prepared 1/4 dilution means 1mi stock and 4ml distilled water Again use the same formula N1 V1 N2 V2 50 1 N24 N2 50/4 12.5 mM is the final concentration of the stock. 0.08-4600 c c 0.08 4500 0.18 micro mole. Is the extinction coetficient for a molecuie the sae fat afi wavelergths) to Beer Lamberf's law A-ecl; where c- concentration of the solution, A- absorbance;length of path followed by light and c molar absorptivity -molar extinction coefficient. Plcase note that 'c' docs not depend on wavelength of light. It depends only on the length of path of light followed and concentration of the solution. Therefore, the extinciton coefficient of the molecule will be same for all