Question

I need help with this lab

tions. Let's review a few resources that will help you prepare for this assignment: What Column Chromatography Looks Like (video) of Column Chromatography (article) . Information on Solvents (web resource) . Pictures of What a Column Looks Like and How It Is Setup (article) A video of .A Column Being Run (video) . Note that the first 4 minutes simply show how the column is prepared. That is not one of your variables. You can assume that you will set up your column the same way. Based on what you have learned in Module 1 and Module 2, devise a column that will separate the following molecules: OH

Answer 1

The given 2 compounds belong to alcohol and ketone family hence they can be separated using extraction
techniques using appropriate solvents. Since the first compound is a simple alkane alcohol it should be soluble
in water and the other ketonic compound should be soluble in methylene chloride. You will observe the
separation of aqueous and organic layer, to increase the effectiveness of separation salts like NH₄Cl can be added.
The organic layer can be washed with alkaline Na₂CO₃ to make it pure. Further TLC can be employed to separate
and identify the compounds effectively.