An experiment was conducted to determine the effects of a novel promoter on the transcription of a gene.
An experiment was conducted to determine the effects of a novel promoter on the transcription of a gene. The cloned promoter and coding regions were ligated to luciferase, a reporter gene. Several regions of DNA in the promoter region were deleted. Each construct was transformed into cells, and the luciferase activity was measured. The data from the experiment is listed in the table.
Which conclusions can be drawn from the data?
-Transcription increases when region A is deleted because the promoter region is located in region A.
-Silencers bind repressors and are located in region C.
-Region D does not contain any response elements.
-Transcription binding sites are not found in region B.
-Basal promoters are located in region D because gene expression decreases when it is deleted.
Homework Answers
Transcription binding sites are not found in region B.
Basal promoters are located in region D because gene expression decreases when it is deleted.
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An experiment was conducted to determine the effects of a novel promoter on the transcription of a gene.
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regulate the expression of a gene that codifies for a protein that
participates in cell division. The gene is called Mitosis
Regulatory Protein A or MRPA. The complete DNA sequences
for the MRPA promoter and coding region have been
identified. However, it is unknown if MRPA has enhancers
regulating its transcription. To answer this question your lab
first produced a transgenic cell line where GFP has been inserted
as a reporter gene....
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