63. In cancer cells one allele of the tumor suppressor gene p53 is frequently mutated so...
63. In cancer cells one allele of the tumor suppressor gene p53 is frequently mutated so that the protein is inactive, not produced, or deleted. The other allele will usually … promoter remains intact but the gene is not expressed. Sequencing with sodium bisulfite modification of DNA can be used to detect which cytosines are methylated. …what would be the anticipated results?
- Cytosines in or near the promoter region will be methylated
- Cytosines in or near the promoter will not be methylated
- Cytosines in the coding region will have an increased methylation
- There will be no differences in the methylation pattern of the promoter of p53 from a cancer cell and a normal cell
64. Genomic DNA is isolated from a bacterial strain. After a long digestion with a 6-base cutting enzyme that recognizes the sequence GAATTC, the bacterial DNA is not … size is 4 Mb (10^6 base pairs=1 megabase), so there should be approximately 1000 sites in the DNA. What is the most likely explanation for this bacterial DNA being …
- There are not Gs in the genome
- There are not As in the genome
- The bacterium methylated its DNA because this bacterial strain produces the 6 base cutting enzyme
- All the recognition sites are in genes, and the enzyme will not cut genes
- All the recognition sites are in promoters and regulatory sequences, and the enzyme cuts only in genes
65. There are “promiscuous plasmids,” plasmids that can be interchanged between very different bacteria. These often pick up transposons carrying antibiotic resistance. …outbreak of a variety of bacteria carrying resistance to streptomycin, gentamicin, and penicillin. How could we determine how this was occurring?
- Test all the resistant bacteria for a plasmid
- See if the same plasmid could be isolated form all resistant bacteria
- See if the plasmid isolated form the bacteria conferred resistance to a test bacterium
- …
Homework Answers
63.Option A
64.Option E
65.All options are not given in the question
From the above options C is the answer
Add Answer to:
63. In cancer cells one allele of the tumor suppressor gene p53
is frequently mutated so...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
4. p53 is an important cancer suppressor gene. To study its function, it is very useful to have p53 knock-out mice. How can one best prepare a strain of p53 knock-out mice? Please start with the optim...
4. p53 is an important cancer suppressor gene. To study its function, it is very useful to have p53 knock-out mice. How can one best prepare a strain of p53 knock-out mice? Please start with the optimal method to introduce mutations into DNA, and then describe how you would incorporate such DNA into the mouse genome. List all major steps. please give detailed answer, thank you!
You have isolated a DNA fragment containing a human gene involved in lung cancer and are...
You have isolated a DNA fragment containing a human gene involved in lung cancer and are working inserting it into a standard plasmid vector used in molecular cloning which has a multiple cloning site in the lacZ gene. After cutting the DNA fragment and the vector with the same restriction enzyme and ligating the cut DNA, you transform bacteria with hopefully the recombinant vector. How do you expect to identify bacteria with the recombinant vector? A. Bacteria with the recombinant...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin?...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin? (A-E) A E പറ B D с 2. Aeromonas hydrophila, the bacterium with this plasmid, was found growing in what organism? 3. The qach and qacEA1 genes provide resistance to what group of disinfectant?? 4. The tetA and tetR genes provide resistance to what type of antibiotics? Aeromones hydrophile plasmid R18 165.906be GC 52.53% 5.. Name the type of horizontal gene transfer diagrammed below....
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
please answer All the multiple choice questions in the pic (all pics) i dont need a...
please answer All the multiple choice questions in the pic (all
pics) i dont need a explantion .
22 Using a bacteriophage to pass DNA rom bacterium to another O A) Transduction O B) Transformation C) Translocation O D) Translation 23. What research did Rosalind Franklin contribute to the elucidation of the double helix structure of DNA? O O O A) Principles of base pairing B) Biochemical data C) Bacterial transformation data D) X ray crystallography A segment of DNA...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration;...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
explain 1 or 2 molecular details in how epigenetics may allow melanoma cells to outsmart BRAF inhibitors **This is for a genetics course Seope: This science commentary claims that epigeneties pl...
explain 1 or 2 molecular details in how epigenetics may allow
melanoma cells to outsmart BRAF inhibitors
**This is for a genetics course
Seope: This science commentary claims that epigeneties plays a role in cancer cells becoming therapy-resistant. The language used is generalized for the non-scientist and does not include any molecular details, such as methylation of a specific nucleotide in an allele or modification of a specific histone tail amino acid. Your task (individually or in a group of...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin? (A-E) A E പറ B D с 2. Aeromonas hydrophila, the bacterium with this plasmid, was found growing in what organism? 3. The qach and qacEA1 genes provide resistance to what group of disinfectant?? 4. The tetA and tetR genes provide resistance to what type of antibiotics? Aeromones hydrophile plasmid R18 165.906be GC 52.53% 5.. Name the type of horizontal gene transfer diagrammed below....
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
please answer All the multiple choice questions in the pic (all
pics) i dont need a explantion .
22 Using a bacteriophage to pass DNA rom bacterium to another O A) Transduction O B) Transformation C) Translocation O D) Translation 23. What research did Rosalind Franklin contribute to the elucidation of the double helix structure of DNA? O O O A) Principles of base pairing B) Biochemical data C) Bacterial transformation data D) X ray crystallography A segment of DNA...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
explain 1 or 2 molecular details in how epigenetics may allow
melanoma cells to outsmart BRAF inhibitors
**This is for a genetics course
Seope: This science commentary claims that epigeneties plays a role in cancer cells becoming therapy-resistant. The language used is generalized for the non-scientist and does not include any molecular details, such as methylation of a specific nucleotide in an allele or modification of a specific histone tail amino acid. Your task (individually or in a group of...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
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