Question

(a) How is the activity of beta-galactosidase useful in bacterial gene cloning?

(b) How does this use for beta-galactosidase differ from drug selection using antibiotics like ampicillin? Give at least a 2-sentence answer for each part.

Answer 1

Ans 1. Beta -Galactosidase helps in catalyzing of the degradation of galactosylceramide to galactose It also shows the activity against galactosylsphingosine and lactosylceramide (both are enzymes

)if I say then This enzyme is located in the suicidal bags of cell as the lysosomes , which within cells to break down and recycle the different types of molecules.

Here recycling means coding or replications of genes which helps in cloning ...

Useful

Beta-galactosidases exist naturally in many organisms, including plants, microbes and animals. For example

the enzymes isolated from bacteria with neutral optimum pH, were used in milk products.

A gene of β-galactosidase from the bacteria Alteromonas hence (dna) sequencing showed that it consisted of encoding with 1058 amino acid residues So, it is therefore cloned from strain and showed that given bacteria shares a conserving activity site and nucleophilic site found in this family of enzyme.

It replicates its by the activity that's useful for cloning. The most important thing that there must be an optimum pH range for enzyme activity and stability was consistent with bacterial β-galactosidases which are normally active within a neutral pH range of 6 to 8.

Ans 2

Ampicillin is an antibiotic useful which inhibits the growing medium which prevents bacteria cloning during the transformation from growing. Hence it binds to and stops several enzymes that are involved in the synthesis of the cell wall.

This antibiotic slowing down this activity of enzyme so as to lower the release to prevent from bacterial prevention ...

Where beta galactosidase has a high specificity for the desired antibiotic , lack of interference by other compounds.

That's why ampicillin is preferred  

Read it carefully I tried to make it short but it comes after reading of actual content .

Hope it clears thumbs up!