Strain WT, genotype MAT (alpha) trp1 ura3 gal2, grows happily on YPD because this is a rich medium. If we wanted to grow WT on minimal media that contained just some glucose, a nitrogen source and some trace elements, is there anything else we would need to add?
There is no need for additional nutrients since minimal medium supports the growth of yeast. Minimal medium is usually used to test the mating type (MAT) of yeast cells.
Strain WT, genotype MAT (alpha) trp1 ura3 gal2, grows happily on YPD because this is a...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...