Homework Answers
The entering of further oxygen will end up in spoiling of the results. So we have to insert and remove the needle along the same line so no oxygen is entered further.
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2 When inoculating agar deep stabs, why do you have to insert and remove the needle...
Questions 1. Why do we see growth of a strict aerobe along the stab line of...
Questions 1. Why do we see growth of a strict aerobe along the stab line of an agar deep? 2. Where in an agar deep would you expect to find the lowest levels of oxygen? The highest? SSVVUOVOVOU 3. What is the role of oxygen in aerobic respiration? 4. Why do facultative anaerobes grow better in an aerobic environment than an anaerobic environment? 5. In an agar deep, how would you distinguish the growth pattern of an aerotolerant anaerobe from...
8. Why did you use an inoculating loop instead of a needle to make the transfers...
8. Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes? 9. How do the pure broth cultures differ? The slant cultures? 10. What is the function of sterile mineral oil in the maintenance of stock cultures? 11. Describe how a culture can be lyophilized. 12. How can some anaerobes be maintained in pure cultures? 13. What are some signs of growth in a liquid medium? 14....
Scenario 2 Your patient complains of serious pain when you insert the needle into the vein. Explain the steps on how you...
Scenario 2 Your patient complains of serious pain when you insert the needle into the vein. Explain the steps on how you would improve the patient's comfort? Your patient asks you how long you have been drawing blood and whether you are "good"? How do you respond? Justify your response.
60. What is used to inoculate the media in a SIM test? a. Inoculating loop b....
60. What is used to inoculate the media in a SIM test? a. Inoculating loop b. Inoculating needle c. Sterile serological pipette d. Sterile cotton swab 61. How many different test results can you get from 1 SIM test? a. 1 b. 2 c. 3 d. 4 62. How can SIM medium be used to assess motility? a. Growth radiates outward in all directions from the stab line and on the surface b. Media turns black c. Formation of a...
Background Background continued... Questions Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Why do you use a bacterial culture plate when working with viruses? How do you count...
Why do you use a bacterial culture plate when working with viruses? How do you count phages? Describe the general process and the end result. If there were no plaques on your plate, offer an explanation. In this procedure, why is it important to use a hard agar with a soft agar overlay to demonstrate plaque formation?
Why might you expect facultative anaerobes to accomplish anaerobic respiration in peptone-iron agar deeps when they...
Why might you expect facultative anaerobes to accomplish anaerobic respiration in peptone-iron agar deeps when they are not incubated in an anaerobic chamber?
When you insert a dielectric into a capacitor, in which case do you do positive work;...
When you insert a dielectric into a capacitor, in which case do you do positive work; when the capacitor is connected to a battery or when it is disconnected from a battery (or in both cases or neither)? Provide your reasoning to support your answer.
E. The patient attempts to jerk her arm away as you are inserting the needle. CHAPTER...
E. The patient attempts to jerk her arm away as you are inserting the needle. CHAPTER 26 I ARTERIAL BLOOD GAS SAMPLING 425 You have reangled the needle three times and are still unable to get the blood sample. G. You notice swelling under the puncture site when you remove the needle. H. As you are corking the needle, you puncture your fingertip. Why does the IV bag in an arterial line setup need to be presurized 6. You are...
1. What is the significance of producing isolated bacterial colonies on an agar plate? 2. Briefly,...
1. What is the significance of producing isolated bacterial colonies on an agar plate? 2. Briefly, explain how the formation of a bacterial colony occurs on an agar plate. 3. Why must you flame the loop between each streak when performing the streak plate techniques? 4. How can you determine whether a culture that you have been provided with is a "pure" culture? 5. You streak a culture and find that the colonies in Quadrant 4 are larger in diameter...
Questions 1. Why do we see growth of a strict aerobe along the stab line of an agar deep? 2. Where in an agar deep would you expect to find the lowest levels of oxygen? The highest? SSVVUOVOVOU 3. What is the role of oxygen in aerobic respiration? 4. Why do facultative anaerobes grow better in an aerobic environment than an anaerobic environment? 5. In an agar deep, how would you distinguish the growth pattern of an aerotolerant anaerobe from...
8. Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes? 9. How do the pure broth cultures differ? The slant cultures? 10. What is the function of sterile mineral oil in the maintenance of stock cultures? 11. Describe how a culture can be lyophilized. 12. How can some anaerobes be maintained in pure cultures? 13. What are some signs of growth in a liquid medium? 14....
60. What is used to inoculate the media in a SIM test? a. Inoculating loop b. Inoculating needle c. Sterile serological pipette d. Sterile cotton swab 61. How many different test results can you get from 1 SIM test? a. 1 b. 2 c. 3 d. 4 62. How can SIM medium be used to assess motility? a. Growth radiates outward in all directions from the stab line and on the surface b. Media turns black c. Formation of a...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
E. The patient attempts to jerk her arm away as you are inserting the needle. CHAPTER 26 I ARTERIAL BLOOD GAS SAMPLING 425 You have reangled the needle three times and are still unable to get the blood sample. G. You notice swelling under the puncture site when you remove the needle. H. As you are corking the needle, you puncture your fingertip. Why does the IV bag in an arterial line setup need to be presurized 6. You are...
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