Imagine that during the serial dilutions/spread plate procedure that your lab partner chose the wrong micropipet...
Imagine that during the serial dilutions/spread plate procedure
that your lab partner chose the wrong micropipet to transfer
samples during the dilution. As a result, he/she ended up
transferring 10 µl instead of 100 µl of culture in the last three
steps. Therefore, the 1/1,000, 1/10,000, and 1/100,000 dilutions
were actually much more diluted than expected. (Don’t yell at your
lab partner.) Based on your partner’s mistake a) recalculate the
actual dilutions and b) recalculate the concentration of cells from
your stock culture.
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Imagine that during the serial dilutions/spread plate procedure
that your lab partner chose the wrong micropipet...
data given Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml...
data given
Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml I ml Tube 11 Tube 21 Tube 3 Tube 4 Il ml 1 ml ml No of bacterial Colonies TNTC TNTC 290 35 Using the Pour-Plate Method What was the purpose of this lab exercise? Complete the following table with your observations: Dilution factor Comments Number of bacteria per 1 ml of original milk Number of colonies on the plate 10 100 1,000 10,000...
In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates...
In our experiment we made 10-fold
serial dilutions of a sample. We made both spread plates and pour
plates. These are our final results from last semester.
Decide which of the following statemtents are true
statements.
QUESTION 6 In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates and pour plates. These are our final results from last semester. Dilution Factor Pour plates Spread Plates TMTC (300+) TMTC (300-) 10-2 TMTC (300+) 10-3 291...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
For part 1 of this lab) I collected a soil sample from my campus Part 2)...
For part 1 of this lab) I collected a soil sample from my campus
Part 2) Tested bacteria initial viability Part 3) DNA extraction
Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing
Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA
sequence data analysis (sent sample to another lab)
Directions for Part 3 DNA extraction are in the attached
image
QUESTIONS REGARDING PART 3 (DNA extraction)
1) What type of conclusions can be made from initially...
data given
Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml I ml Tube 11 Tube 21 Tube 3 Tube 4 Il ml 1 ml ml No of bacterial Colonies TNTC TNTC 290 35 Using the Pour-Plate Method What was the purpose of this lab exercise? Complete the following table with your observations: Dilution factor Comments Number of bacteria per 1 ml of original milk Number of colonies on the plate 10 100 1,000 10,000...
In our experiment we made 10-fold
serial dilutions of a sample. We made both spread plates and pour
plates. These are our final results from last semester.
Decide which of the following statemtents are true
statements.
QUESTION 6 In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates and pour plates. These are our final results from last semester. Dilution Factor Pour plates Spread Plates TMTC (300+) TMTC (300-) 10-2 TMTC (300+) 10-3 291...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
For part 1 of this lab) I collected a soil sample from my campus
Part 2) Tested bacteria initial viability Part 3) DNA extraction
Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing
Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA
sequence data analysis (sent sample to another lab)
Directions for Part 3 DNA extraction are in the attached
image
QUESTIONS REGARDING PART 3 (DNA extraction)
1) What type of conclusions can be made from initially...
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