To achieve the calibrated measurement of the sample, multiply the measured value of the sample by the labeled magnification of the objective lens divided by the actual magnification ratio. An ocular micrometer is a glass disk with ruled scale on the surface which is etched in chromium.
When you calibrated your eyepiece micrometer, you determined how many microns (µM) each eyepiece unit spans...
The length of a stage micrometer is 1 mm and it is marked with 100 tic marks including 10 long tic marks with number labels (0.1, 0.2, 0.3, etc.). The eyepiece (ocular) graticule has 50 tic marks, with a longer tic mark at every 10th position (meaning there are 5 groups of 10 units = 50 eyepiece graticule units). When the stage micrometer under the 10x objective lens is lined up with the eyepiece graticule so that the tic marks...