What is the plate height for a 10.0-cm HPLC column packed with 3.5 μm solid phase?
What is the plate height for a 10.0-cm HPLC column packed with 3.5 μm solid phase?
A peak elutes from an HPLC column 11.8-cm in length in 13.8 min. What would be the width at half-height of the peak (in seconds) if the plate height were: (a) 6.9 μm? (b) 36 μm?
A peak elutes from an HPLC column 14.9 cm in length in 15.5 min What would be the width at half‑height of the peak (in seconds) if the plate height were 5.64 μm? (My answer: w1/2= 13.44s this was correct; I only need the next question; below) What would be the width at half‑height of the peak (in seconds) if the plate height were 38.0 μm? ?1/2=_s
A HPLC column was operated under the following conditions
Column Length – 25.4 cm Column
Diameter = 0.5 mm
Mass stationary phase = 0.49
g Density
Stationary Phase = 1.12 g/mL
Measured Outlet Flow Rate = 1.0 mL/min
Column Temperature = 25 oC.
resolution equation=Rs = Trb-Tra/W
Plate height(H) = length of column (L)/Number of plate on the
column (N)
No. of theoretical N= 16(RS/w)2
Calculate the resolution for components #3 and #4?
Calculate the plate height for column (use...
4) What is the order of elution of the following compounds from a reverse-phase packed HPLC column? Briefly justify your reasoning. Benzyl alcohol (C6H5CH2OH), benzaldehyde (C6H5CHO), benzoic acid (C6H5COOH), Acetophenone (C6H5C(O)CH3)
A peak elutes from an HPLC column 17.3 cm in length in 14.0 min. What would be the width at half-height of the peak (in seconds) if the plate height were 4.83 um? W1/2 = What would be the width at half-height of the peak (in seconds) if the plate height were 39.0 um? W1/2 =
3. (2 pts) Why is eddy diffusion a larger contribution to plate height in packed column gas chromatography than in liquid chromatography?
When packing a column, normally to about what height should the column be packed with adsorbent? A. to a height equal to one-fourth the mass of the solvent B. to a height about 10 times the diameter of the column being use C. about 4 centimeters below the top of the column D. about 18 centimeters up from the bottom
What particle diameter is needed to give 15 000 plates over a 10-cm HPLC column?
A GC column is operated under the following conditions: Column: 1.10 m x 2 mm packed with Chromosorb P and Carbowax 20M as stationary phase. The following retention times were obtained: air: 18.0 sec; methylacetate: 2.98 min; methyl propionate: 4.56 min. Peak width at half height: methylacetate: 7.7 sec; methyl propionate: 12.4 sec. Calculate: a) the corrected retention time for each solute. b) the capacity factor k' for each solute. c) the selectivity factor α for the pair of solutes....
1) why does pH affect the HPLC separation of vitamins on a reverse phase column? 2) considering the Van Deemter equation, what effect would decreasing the particle size have on resolution? please answer question number 2!