What method is used to inoculate Kligler iron agar? Question 4 options: Placing 3 loops of...
What method is used to inoculate Kligler iron agar?
Question 4 options:
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Placing 3 loops of bacteria into the liquid |
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Smearing a spot on the agar plate |
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Streaking the agar surface |
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Streaking the agar surface and stabbing into the agar |
Homework Answers
The method which is used to inoculate kligler iron agar is.
D. Streaking the agar surface and stabbing into the agar
First, from a pure culture, stabbing a culture by inoculation loop and then streak the agar surface in sterile conditions.
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What method is used to inoculate Kligler iron agar?
Question 4 options:
Placing 3 loops of...
QUESTION 6 Why is agar used in lab, give important properties of agar AND doncribe why...
QUESTION 6 Why is agar used in lab, give important properties of agar AND doncribe why it would be important to separate two species of bacteria on by spreading the across a plate as was done in the lab dass TTT Arial 3(126 T E . .
1. What are 4 standard terms used to describe morphological characteristics of colonies growing on an...
1. What are 4 standard terms used to describe morphological characteristics of colonies growing on an agar plate? 2. Describe the T-streak method 3. Describe which inoculation tool is the most approproiate to use when transfering bacteria from an agar plate to a stab tube. 4. Why is a buffer added to agar? Explain your answer. 5. Look up a growth curve for bacteria growing on an agar plate. Describe why the lag phase occurs.
Background Background continued... Questions Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Question 7 (1 point) Saved After steaking a sheep blood agar plate with a throat swab,...
Question 7 (1 point) Saved After steaking a sheep blood agar plate with a throat swab, the purpose of stabbing the inoculum inot the agar (or placing in an anaerobic jar) is to: O provide reduced oxgyen tension for better growth obtain isolated colonies of respiratory flora clean off the inoculating loop enhance the beta-hemolytic activity of the pathogen - Question 11 Respiratory culture has the folloiwng results on BAP. 1. Alpha-hemolytic 2. G (+) lancet shaped cocci 3. catalase...
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4....
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4. For what purpose do bacteria that have the enzyme utilize it? 6. What will occur if the plate is incubated right side up? 7. What will occur if the plate is incubated for more than 24-48 hours? Exercise 7: Selective and Differential Growth Medium and Tests 95 Exercise 7.9 Hydrolytic Exoenzymes: Lipase and Casease LIPASE TEST Lipids are fatty acids used by some bacterium...
“3. What ingredient(s) makes MacConkey agar differential?” Click here to enter text. “4. Why is an...
“3. What ingredient(s) makes MacConkey agar differential?” Click here to enter text. “4. Why is an indicator dye used in MacConkey agar?” Click here to enter text. “5. What are some potentially pathogenic bacteria that are lactose fermenters that will grow on MacConkey agar?” Click here to enter text.
60. What is used to inoculate the media in a SIM test? a. Inoculating loop b....
60. What is used to inoculate the media in a SIM test? a. Inoculating loop b. Inoculating needle c. Sterile serological pipette d. Sterile cotton swab 61. How many different test results can you get from 1 SIM test? a. 1 b. 2 c. 3 d. 4 62. How can SIM medium be used to assess motility? a. Growth radiates outward in all directions from the stab line and on the surface b. Media turns black c. Formation of a...
D | Question 3 0.25 pts What will be the color of the agar if Staphyłococcus...
D | Question 3 0.25 pts What will be the color of the agar if Staphyłococcus epidermidis grows on MSA plate? O Red O White D Question 4 0.25 pts If 1 gm of food sample was added into 99 ml of diluent, then what will be the dilution factor for the food sample? 0 10 dilution 102 dilution 10 dilution 10t dilution 0.25 pts D Question 5
QUESTION 4 Why is media such as Sabouraud Dextrose Agar (SDA) used for the growth of...
QUESTION 4 Why is media such as Sabouraud Dextrose Agar (SDA) used for the growth of yeast and fung? Choose all that apply. lower pH inhibits growth of bacteria is differential for bacteria and fungi/yeasts QUESTIONS The difference between MBC and MIC is that... the MBC is the highest concentration of antibiotic that kills the organism will the MIC is the lowest concentration of antibiotic that kills the organism. The MBC is the lowest concentration of antibiotic that inhibits bacterial...
Question 1 p Flag question Not yet answered Marked out of 1 What is an extraction?...
Question 1 p Flag question Not yet answered Marked out of 1 What is an extraction? Select one: a. Separation of compounds in a mixture based on their solubility in miscible solvents. b. Separation of compounds in a mixture based on their solubility in immiscible solvents. Question 2 Not yet answered Marked out oft Flag question In a liquid-liquid extraction the compound (or solute) prefers to be dissolved in one liquid versus the other liquid. Select one: True False Question...
QUESTION 6 Why is agar used in lab, give important properties of agar AND doncribe why it would be important to separate two species of bacteria on by spreading the across a plate as was done in the lab dass TTT Arial 3(126 T E . .
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Question 7 (1 point) Saved After steaking a sheep blood agar plate with a throat swab, the purpose of stabbing the inoculum inot the agar (or placing in an anaerobic jar) is to: O provide reduced oxgyen tension for better growth obtain isolated colonies of respiratory flora clean off the inoculating loop enhance the beta-hemolytic activity of the pathogen - Question 11 Respiratory culture has the folloiwng results on BAP. 1. Alpha-hemolytic 2. G (+) lancet shaped cocci 3. catalase...
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4. For what purpose do bacteria that have the enzyme utilize it? 6. What will occur if the plate is incubated right side up? 7. What will occur if the plate is incubated for more than 24-48 hours? Exercise 7: Selective and Differential Growth Medium and Tests 95 Exercise 7.9 Hydrolytic Exoenzymes: Lipase and Casease LIPASE TEST Lipids are fatty acids used by some bacterium...
60. What is used to inoculate the media in a SIM test? a. Inoculating loop b. Inoculating needle c. Sterile serological pipette d. Sterile cotton swab 61. How many different test results can you get from 1 SIM test? a. 1 b. 2 c. 3 d. 4 62. How can SIM medium be used to assess motility? a. Growth radiates outward in all directions from the stab line and on the surface b. Media turns black c. Formation of a...
D | Question 3 0.25 pts What will be the color of the agar if Staphyłococcus epidermidis grows on MSA plate? O Red O White D Question 4 0.25 pts If 1 gm of food sample was added into 99 ml of diluent, then what will be the dilution factor for the food sample? 0 10 dilution 102 dilution 10 dilution 10t dilution 0.25 pts D Question 5
QUESTION 4 Why is media such as Sabouraud Dextrose Agar (SDA) used for the growth of yeast and fung? Choose all that apply. lower pH inhibits growth of bacteria is differential for bacteria and fungi/yeasts QUESTIONS The difference between MBC and MIC is that... the MBC is the highest concentration of antibiotic that kills the organism will the MIC is the lowest concentration of antibiotic that kills the organism. The MBC is the lowest concentration of antibiotic that inhibits bacterial...
Question 1 p Flag question Not yet answered Marked out of 1 What is an extraction? Select one: a. Separation of compounds in a mixture based on their solubility in miscible solvents. b. Separation of compounds in a mixture based on their solubility in immiscible solvents. Question 2 Not yet answered Marked out oft Flag question In a liquid-liquid extraction the compound (or solute) prefers to be dissolved in one liquid versus the other liquid. Select one: True False Question...
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