A researcher identified some mutant barley plants that had unusually elongated internodes in their stems and their seeds lacked dormancy. She found that she could reproduce these phenotypes in wild type plants or seeds by applying a plant hormone. What growth regulator do you think this was? What might be the cause of the mutation she discovered? How could you test your hypothesis?
One of the most important functions of auxin is internode elongation. In the question, it is given that the mutant has abnormally long internode. It means the mutant maybe over expressing the gene which is encoding for auxin.
The hypothesis can be tested -
1. Collect 10 wild type plants.
2. Prepare auxin solution at different concentration, ranging from 0.1 micromole to 1 micromole.
3. Prepare a semisolid plant nutrient medium. Prepare 22 such test tubes.
4. Take 2 tubes as control which will not have auxin.
5. Prepare 20 tubes for 0.1 to 1 micromole auxin. Each tube will be in duplicate.
6. Innoculate the wild type plant in the test tube having medium (and auxin).
7. Incubate at appropriate temperature and humidity. Take the readings of the length of internode at this day and then take the length after every 3 days.
8. Maintain this experiment for at least one month.
You will be able to find out with auxin concentration was the most effective in elongating the internode of the given plant.
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A researcher identified some mutant barley plants that had unusually elongated internodes in their stems and...
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