Question

Please remember to provide the reference(s) used to answer any
question—include it at the end of your answer. For journal article references, give
the name of the article, all authors, the journal, volume, and page numbers. For a
website reference (do not use Wikipedia,) provide the full name of the URL and data
accessed. Please type in answer in TEXT so it can be clear!

3. Give one explanation for why you may have obtained a false negative result in the
actual experiment conducted in lab.
4. Describe an ELISA procedure that you would use to determine if the potatoes from a
particular supermarket supplier had been infected with a tuber virus?

Answer 1

1- False negative results due to improper experiment. Here negative means the (absence of a condition) i.e. the presence of something is not there. But in reality, it is present. You can observe false positive results because of certain experimental inhibitors present in your samples which hinders the positive result and give negative results. Consider a situation of confirming the presence of a virus in your sample, but because your plasma contains RT PCR inhibitors you don't see the amplification of the viral gene. But its there.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC139694/

2- In this case, we can do direct ELISA. It includes bind of the potato samples hypothetically contains virus onto the surface, followed by incubating with the antibody bound with the enzyme that will attach to the viral antigen on the samples over the surface. Then we can add the substrate that with react with the bound enzymes and give the signal. The occurrence of the signal will suggest the viral infection in the potatoes is positive.