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The pKa of carboxyl groups typically fall somewhere between pH2 to pH4. At pH5 the carboxyl...

The pKa of carboxyl groups typically fall somewhere between pH2 to pH4. At pH5 the carboxyl groups of myoglobin should all be in their carboxylate form (COO– ); however, myoglobin does not bind well to DEAE at pH5. Explain why this happens.

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Ans.) Instead of presence of negative charge over protein due to deprotonation carboxylic acid to carboxylate ion at pH 5 to DEAE. The reason for not proper binding could be excessive salt concentration in buffer solution. Salts in buffer compete with the negatively charged protein.

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