Question

Describe the membrane budding reactions that occur throughout the cell. What coats are used where?

Answer 1

protein modification occur in two compartments ER ( N- linked glycosylation ) and Golgi ( o- linked glycosylation).

protein transported from ER to Golgi in anterograde process involving COPII proteins . The vesicle buds from the ER and transported to the golgi ( cis golgi transport) and then finally buds from trans golgi network and reach the destination.

protein from golgi to ER move in retrograde process involving COPI proteins.

The soluble proteins enter the cell through endocytosis and enter the vesicle coated with clathrin , the clathrin coated vesicle buds from the plasma membrane and then uncoating take palce and finally the fusion of the membrane occur by v- SNARE and t- SNARE hypothesis. clathrin transport the protein from plasma membrane to lysosome.

protein synthesized by free ribosomes move to peroxisomes, mitochondria and chloroplast.

protein synthesized by membrane bound ribosomes moves to ER , golgi and lysosome.