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Given a 0.200 M solution of anserine at its isoelectric point and ready access to 0.100...

Given a 0.200 M solution of anserine at its isoelectric point and ready access to 0.100 M HCl, 0.100 M NaOH and distilled water, describe the preparation of 1 L of 0.0500 M anserine buffered solution, pH 7.20.

Volume of stock solution needed =____ mL

Volume of HCl needed to adjust the imidazole group =____ mL

Volume of HCl needed to titrate the amino group =_____ mL

Total HCl needed = _____ mL

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