Which of these haploid strains produce permease but do not produce β-galactosidase? A) I- P+ O+ Z+ Y+ B) I+ P+ O+ Z-Y+ C) I- P+ O+ Z-Y D) I+ P+ O- Z+ Y+ E) I- P+ O+ Z+ Y+
Which of these haploid strains produce permease but do not produce β-galactosidase? A) I- P+ O+...
For each genotype, with and without inducer, select whether or not a functional galactosidase or permease will be present. IS indicates a super-repressor (it can’t bind to lactose/inducers). OC indicates a constitutive operon (no repressor can bind to this region of DNA). I- ; Z- ; Y- each indicate an allele that does not produce a functional protein. P- indicates a mutation in the promoter region such that RNA polymerase does not bind. Group of answer choices I+ P+ O+...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence or absence of lactose, as indicated below. The presence (+) or absence (-) of β-galactosidase and permease expression are investigated in these cultures. Which of the following options (A-E) would be the expected results for bacteria with the genotype IS P+ O+ Z+ Y- / I+ P+ OC Z– Y+ ? β-galactosidase Permease No lactose Lactose No lactose Lactose A - + - +...
The lac operon consists of a promoter that initiates
transcription of the gene i, which encodes a repressor protein. A
seperate promoter, p, within the operon initiates transcription of
z, which encodes B-galactosidase, and y, which encodes permease.
The operator, o, where the repressor protein binds to inhibit
transcription, is positioned between mutations, whereas superscript
c denotes a constitutively active mutant. The table below sea fives
the activity of B-galatosidase and permease for haploid and diploid
mutant strains. Match the...
1- For each genotype, with and without inducer, select whether or not a functional galactosidase or permease will be present. IS indicates a super-repressor (it can’t bind to lactose/inducers). OC indicates a constitutive operon (no repressor can bind to this region of DNA). I- ; Z- ; Y- each indicate an allele that does not produce a functional protein. P- indicates a mutation in the promoter region such that RNA polymerase does not bind. ----------------------------------------------------------------------------------------------------------------- I+ P+ OC Z+ Y+...
which operon will make b-galactosidase even if lactose
isn't present?
p+ o+ lacZ+ lacI-
p- Oc lacZ+ lacI-
p+ Oc lacZ- lacI+
p- o+ lacZ+ lacI+
p+ o+ lacZ+ lacI+
Oc = fails to bind R
regulatory promoter operator gene structural genes lacz lacY lacA β-galactosidase thiogalactoside transacetylase lactose permease
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Absent in both the presence and absence of lactose; (B) Abundant only in the absence of lactose; (C) Abundant only in the presence of lactose; (D) Abundant in both the presence and absence of lactose; or (E) Abundant only in the presence of glucose. I. i(-); II. p(-)o(c); III. i(-)p(+) / i(+)p(-); IV. i(s)p(+)o(c) / i(+)p(-)o(+).
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
The lac operon contains three genes: lacZ, lacY, and lacA for β‑galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively. The operon also contains a promoter site and an operator site. The regulatory gene includes the I gene for the Lac repressor protein and a promoter for that gene.The lac operon undergoes negative regulation. In the normal condition, the Lac repressor protein is active. Allolactose, an isomer of lactose, is the signal molecule that binds to the Lac repressor. Determine which events...