Question

Which of the following is correct order to process the growing colonies:

1. plasmid purification

2. inoculate liquid culture

3. perform agarose gel electrophoresis

4. set up restriction enzyme digest

A. 1,2,3,4

B. 2,1,4,3

C.1,2,4,3

Answer 1

The correct answers is ,

B.2,1,4,3

Screening by restriction digestion is the classical method to determine the recombinant colonies by performing inoculating in a liquid culture and a plasmid miniprep followed by restriction digestion.This technique is based on the principle that if the transformed colonies contain the insert of interest it can be digested from vector with the help of restriction enzyme and can be analysed by Agarose gel electrophoresis.