Question

5. Circle all components of translation that dissociate upon the action of release factors (e.g. RF1/2 and RF3). 2 pts

a. deacylated tRNA b. ribosomal subunits c. acylated tRNA

d. polypeptide e. peptidyl transferase

6. GCN4 is a protein whose production is regulated at the level of translation by phosphorylation of the eIF2 α subunit.

a. What interaction is affected when eIF2 is phosphorylated by an eIF2α kinase? 2 pts

a. The interaction between eIF2 and a tRNA.

b. The interaction between eIF2 and the small subunit.

c. The interaction between eIF2 and eIF2B.

d. There is no interaction between eIF2 and its GAP protein.

7. What is the function of GCN4? 2 pts

a. GCN4 is a eukaryotic translation initiation factor which activates translation of amino acid synthesis enzymes.

b. GCN4 is a transcriptional activator that activates expression of enzymes that synthesize amino acids.

c. GCN4 causes termination of translation.

d. GCN4 facilitates exchange of GDP for GTP for many different types of translation factors in eukaryotes.

e. GCN4 is the set of eukaryotic initiation factors that bind to an mRNA.

Answer 1

5. components of translation that dissociate upon the action of release factors (e.g. RF1/2 and RF3):

c. acylated tRNA

d. polypeptide

6. GCN4 is a protein whose production is regulated at the level of translation by phosphorylation of the eIF2 α subunit. the affected interaction when eIF2 is phosphorylated by an eIF2α kinase is:

c. The interaction between eIF2 and eIF2B.

7. Function of GCN4:

b. GCN4 is a transcriptional activator that activates expression of enzymes that synthesize amino acids.