Describe how to activate (turn on) the GFP gene as well as the lac operon. What...
Describe how to activate (turn on) the GFP gene as well as the lac operon. What are the functions of restriction enzyme?
Homework Answers
Green Fluorescent Protein gene is a protein composed of 238 Aminoacid residues that exhibits green fluorescence when exposed to light of blue to UV range.
Lac operon is activated by the presence of lactose and absence of glucose.
Functions of restriction enzymes is to cleave DNA at specific sites along the molecule.
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Describe how to activate (turn on) the GFP gene as well as the
lac operon. What...
7. How does a repressor protein turn off the lac operon? 8. How does lactose turn...
7. How does a repressor protein turn off the lac operon? 8. How does lactose turn on the lac operon? 9. Complete the table to describe the role of each regulatory region or molecule in the operation of the lac operon.
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of lac operon in E.coli. b. Role of CAP and cAMP the activator binding site c. Role of Lac Repressor and Operator and Inducer d. Role of Looping e. Effect of mutations f. Recessive v dominant mutations g. Complementing v noncomplementing mutations. Describe the regulation of the trp operon in E. coli. Describe at least three kinds of molecular switches involved in gene regulation. What...
Concerning the lac operon in prokaryotic gene regulation, describe what a polycistronic message is and its...
Concerning the lac operon in prokaryotic gene regulation, describe what a polycistronic message is and its significance.
The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
Lac Operon Homework . Unanswered How would a mutation in the lacy gene effect the lac...
Lac Operon Homework . Unanswered How would a mutation in the lacy gene effect the lac operon o A There would be normal levels of lac operon expression O B There would be over expression of the lac operon because it would not be repressible O C There would be under expression of the lac operon because of the absence of the inducer Unanswered
b. What is the polycistron in the Lac operon? In the Trp operon? d. Name an aspect of...
b. What is the polycistron in the Lac operon? In the Trp operon? d. Name an aspect of prokaryotic gene regulation that can only occur in a prokaryote and not a eukaryote e. Provided the wildtype lac operon, give three different examples of mutations, what they target, and how they disrupt WT regulation of the operon. f. Describe how the RNA leader of the Trp operon can repress/induce gene translation
Complete the comparison of the lac Operon and trp Operons as a means of gene regulation.
ACTIVITY #2: Operon control of gene expressionComplete the comparison of the lac Operon and trp Operons as a means of gene regulation. lac operorn trp operon Regulates production of Name of Regulatory gene" and what it produces What binds to the operator & when does this occur: Structural genes controlled by the operon and how their expression is controlled: High levels of what substance affects the operon how?
Describe and label the regions on the lac operon. Include the following: regulatory gene, promoter region,...
Describe and label the regions on the lac operon. Include the following: regulatory gene, promoter region, operator, structural genes, and repressor. What happens in the presence of glucose? Lactose?
15. Describe the gene expression at the lac operon in the following conditions. Please discuss all...
15. Describe the gene expression at the lac operon in the following conditions. Please discuss all components involved and how they regulate the expression: a. Absence of glucose; presence of lactose b. Absence of lactose; presence of glucose
1. Describe in detail how both positive and negative regulation control expression of the lac operon...
1. Describe in detail how both positive and negative regulation control expression of the lac operon in bacteria. In doing this, also convey understanding of how and why expression of this operon is controlled two ways (positive and negative). Also answer, what are the energetic benefits to the cell of operon regulation? 2. What is horizontal gene transfer? Describe in detail the three mechanisms of horizontal gene transfer. What are the evolutionary advantages and costs of horizontal gene transfer. I...
7. How does a repressor protein turn off the lac operon? 8. How does lactose turn on the lac operon? 9. Complete the table to describe the role of each regulatory region or molecule in the operation of the lac operon.
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
Lac Operon Homework . Unanswered How would a mutation in the lacy gene effect the lac operon o A There would be normal levels of lac operon expression O B There would be over expression of the lac operon because it would not be repressible O C There would be under expression of the lac operon because of the absence of the inducer Unanswered
1. Describe in detail how both positive and negative regulation control expression of the lac operon in bacteria. In doing this, also convey understanding of how and why expression of this operon is controlled two ways (positive and negative). Also answer, what are the energetic benefits to the cell of operon regulation? 2. What is horizontal gene transfer? Describe in detail the three mechanisms of horizontal gene transfer. What are the evolutionary advantages and costs of horizontal gene transfer. I...
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