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If lacI is moved in between lacY and lacA will the lac operon be transcibed?

If lacI is moved in between lacY and lacA will the lac operon be transcibed?

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Answer #1

LacI is known as repressor, which binds to the operator sites and prevents the transcription of genes. In the absence of lacI, the transcription of genes occurs. When the lacI is inserted between lacY and lacA structural genes, the transcription of genes all the three genes lacZ, lacY and lacA occurs because the lacI when binds to operator , can inhibit the transcription. As the operator is present at the beginning, so the lacI will not be able to bind to the operator. Therefore , transcription of all three genes will happen.

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