If the X-intercept on a Lineweaver-Burk plot is -0.34 mM-1 what is the Km?
| -0.34 mM-1 |
| 0.34 mM-1 |
| -2.9 mM |
| 2.9 mM |
If the X-intercept on a Lineweaver-Burk plot is -0.34 mM-1 what is the Km? -0.34 mM-1...
How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept) O km = (-1) * (x-intercept) O km = 1/(x-intercept) 0 Km = s;lope
If a Lineweaver-Burk plot gives a line with an equation of y = 0.33 x + 0.55, what are the values of KM and Vmax?
You perform a series of enzyme activity assays and then graph the data using a Lineweaver-Burk plot. You determine the X-intercept is at -0.02 mM-1 and the Y-intercept is at 5.0 (mM/sec)-1. Calculate the Vmax and Km for this enzyme. A. Vmax = 0.20 mM/sec; Km = 50.0 mM B. Vmax = 0.20 mM/sec; Km = ‒50.0 mM C. Vmax = 5.0 mM/sec; Km = 0.02 mM D. Vmax = 5.0 mM/sec; Km = ‒0.02 mM
Below is a Lineweaver-Burk plot of enzyme A. What is the Km of this enzyme? (round the answer to the 3 significant figures) 0.7 0.6 0.5 y =0.4843x+0.1951 0.4 1/v (pmol/L)/min) 0.3 30.2 0.1 0 0 0.1 0.2 0.3 0.5 0.6 0.7 0.8 0.9 0.4 1/[S] (umol/L) 2.50M 2.50 M 2.48 M 248M
4. The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by: 1 Km 1 1 where, the plot of (1/V.) vs (1/[S]) is a linear plot. If you only know the x-axis and y-axis intercepts from this plot, how can you determine Vmax and Km? (A) multiply the reciprocal of the x-axis intercept by -1. (B) multiply the reciprocal of the y-axis intercept by -1. (C) take the reciprocal of the x-axis intercept. (D) take...
Why does the Lineweaver-Burk plot give a more accurate Vmax and Km value compared to a Michaelis-Menten plot?
12. From the Lineweaver-Burk graph given: a. What are the Km and Vmax for the uninhibited case? b. What type of inhibition is occurring? c. Shown on the page after the Lineweaver-Burk plot are semi-qualitative sketches for three V vs. (S) plots. Which one of these most likely corresponds to this particular experiment (A,B or C)? Scani explain th Cant catimer IIIIIIIII IIIIIIIII - -- | - | IIIII We were unable to transcribe this image
What is one major disadvantage of using a Lineweaver-Burk plot for determination of kinetic parameters from real data? A.) A Lineweaver-Burk plot biases data with fast initial velocities B.) The Lineweaver-Burk plots tend to be reliant on how reproducible the initial rate is C.) The Lineweaver-Burk plot biases low concentration data D.) all of the above
What is one major disadvantage of using a Lineweaver-Burk plot for determination of kinetic parameters from real data? A.) A Lineweaver-Burk plot biases data with fast initial velocities B.) The Lineweaver-Burk plots tend to be reliant on how reproducible the initial rate is C.) The Lineweaver-Burk plot biases low concentration data D.) all of the above
LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the final reading on your blood glucose meter for one of your samples is 385 mg/dL. You need your data to be in units of mm (millimolar, which is millimoles per liter, abbreviated as mmol/L). Show, in a step-by-step conversion, how you would convert 385 mg/dL to the proper answer in mM. 385 mg dlº= ?mm immoles 2. Assuming that the reaction occurred over a...