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A stock of lytic bacteriophages produced 2000 plaques on a plate when 20 μl was plated...

A stock of lytic bacteriophages produced 2000 plaques on a plate when 20 μl was plated with 0.3 ml of E. coli culture. How many plaques would you expect from a 1:1000 dilution of the same stock plated in the same way?

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Answer #1

For without dilution we are getting = 2000 plaques.

Now doing 1:1000 dilution = 2000 plaques X 1/1000 = 2 plaques.

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