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2a) Explain why steady state currents are expected using amperometry in stirred solution? 2b) Explain how...

2a) Explain why steady state currents are expected using amperometry in stirred solution?

2b) Explain how the response to glucose could be made linear over a wider concentration range?

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n 2b)  ANSWER

Optimisation of the screen printed Electrode the loadings of the electrocatalyst and the Enzyme can be varied. In a study conducted Crouch et al, using chronamperometry, It has been found that the maximum linear range can be acheived with a CoPC loading of 20% m/m relative to the mass of carbon employed in the manufacturing process and a glucose oxidase loading of 628 U per gram of carbon. Optimal operating potential was found to +0.5v following an incubation period of 60s. Additionally an optimum supporting electrolyte was found to be 0.05M Phosphate buffer at pH of 8 which resulted in a linear range of 0.2 to 5mM.

Sensitivity was deemed to be 1.12uA mM^-1 at an optimal operating temperature of 40 degree celsius.

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