Question

1. For 1HPN, a ________ group in each IDS residue indicates the presence of a repeating _________.

a. carboxylate; aldonic acid

b. carboxylate; uronic acid

c. sulfate; aldonic acid

d. sulfate; uronic acid

2. The antibody of 1IGT contains an _____ oligosaccharide that is covalently bound to ________

a. N-linked; Thr 318

b. o-linked; Thr 318

c. o-linked; Asn 314

d. N-linked; Asn 314

3. In the structured presented by 4DUR, the oligosaccharide is attached to the protein by ______ through its _____ atom.

a. o-linkage; anomeric carbon

b. o-linkage; C2

c. N-linkage; anomeric carbon

d. N-linkage; C2

Answer 1

Answers:

1. (b)

Explanation: In the glycosaminoglycan heparin protein structure (PDB: 1HPN), a carboxylate group in each 2-O-sulfo-α-L-iduronic acid (IDS) residue indicates the presence of a repeating uronic acid. (PMID: 8352752)

2. (d)

Explanation: In the structure of IgG2a Monoclonal Antibody (PDB: 1IGT), the oligosaccharide component is N-linked to the heavy chains through Asn314. (PMID: 9048542)

3. (a)

Explanation: In the Type II plasminogen (PDB: 4DUR) the oligosaccharide is attached to the protein by O-linked glycosylation through its anomeric carbon atom on Thr346. (PMID: 22832192)

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