1) GFP tagging is a commonly used method to label cells for visualization by fluorescence microscopy. Which of the following statement is most correct?
a) The GFP tag can be visualized only in fixed cells
b) The GFP tag can only be placed on the N-terminus of a protein
c) The GFP tag can attach to the phospholipids of any membrane
d) The GFP tag allows for visualizing the dynamic behavior of a protein by live imaging
e) The GFP tag disrupts the function of the protein to which it is attached
2) Which of the following statements describes a correct fact about cytoskeletal filaments?
a) Each filament is made up of covalently bound subunits
b) The three cytoskeletal fibers are functionally redundant
c) All three fibers are made up of proteins from the same protein family
d) All three fibers are nucleated at the centrosome (MTOC)
e) All three fibers are assembled from monomeric or dimeric building blocks
Answer-1) GFP is green fluorescent protein and it allows for visualizing the dynamic behavior of a protein by live imaging. So, option (d) is the most correct one.
In first option it is written that it can only be done of fixed cells(dead cells), which is wrong.
The GFP can be fused with any of the N- or C-terminal of a protein hence, option b is incorrect.
Answer 2)There are three types of protein fibers in the cytoskeleton: microtubules, intermediate filament and microfilament. And all the protein fibers have a functional role. Hence, option (b) can be eleminated.
Only microfilament is covalantly bound hence, option (a) is eleminated.
Only microtubules are nucleated at the centrosome. Hence, option (D) is eleminated.
Option (e) is correct. because all three fibers are assembled from monomeric or dimeric building blocks.
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