Question

Answer the following questions:

a) What is special about the E coli BL21(DE3)pLysS cells used for gene expression?

b) In what structural form is the expressed gdhA gene synthesized in the E coli host cells?

c) What physical attributes of SDS-PAGE gels allow the separation and molecular weight determination of polypeptides?

d) Given the size of the gdhA gene of ~1362 bp, what is the theoretical size of the expressed protein?

e) Does your answer from (d) agree with the MW size of your expressed protein, and if not, Why?

Answer 1

The que is not clear