Summarise the difference between linear, circular and supercoiled DNA.
- Summarise the difference between linear, circular and supercoiled DNA.
Homework Answers
Linear DNA is one in which DNA is arranged in the form of multiple molecules with ends. Example in eukaryotes.
Figure: linear DNA
Circular DNA is the one having DNA arranged in the closed loop with no ends. Example , in prokaryotes.
Figure: Circular DNA
Supercoiled DNA is the double helix DNA with additional twist which could be in the same direction or opposite direction. Supercoiling help the DNA to remain compact when not transcribing.
Figure: Supercoiled DNA
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Summarise the difference between linear, circular and
supercoiled DNA.
This figure represents supercoiled, circular, double-stranded DNA: If this molecule of DNA originated as a linear...
This figure represents supercoiled, circular, double-stranded DNA: If this molecule of DNA originated as a linear molecule with a linking number (L) of 30, which was then circularized and unwound, the L for the unwound circular molecule would be O32 O 18 O 28 O 30 20
4. Which form of DNA is possible as well as the linear form? a. circular b....
4. Which form of DNA is possible as well as the linear form? a. circular b. stem loop c. A-DNA d. supercoiled e. B-DNA 5. Why might a single base-pair mutation in eukaryotic mRNA be less serious than one in prokaryotic mRNA? a. If the mutation occurs in the exon, it will not affect the gene product. b. If the mutation occurs in the 3′ end of the start site, it will not affect the gene product. c. If the...
in regards to lane 3 of this gel, which band is nicked, linear and supercoiled? Lysis...
in regards to lane 3 of this gel, which band is nicked, linear
and supercoiled?
Lysis & Boiling Method Preps (Students) 45 ked ectrophoresis ests) wolts a nicked ) linear supercoiled 1% Agarose Gel Graduate Students Alkaline Lysis-Boiling Method Preps EcoRI-BamH-I Digests 02-14-2020 A2188
You discover an organism that has a phospholipid monolayer, tightly supercoiled DNA, and proteins with more...
You discover an organism that has a phospholipid monolayer, tightly supercoiled DNA, and proteins with more Beta-sheets and alpha-helices. Based on these observations, this organism would likely thrive (not simply survive) in--------? a. Alkaline lake b. Salt marsh c. glacier d. hot springs
You are given a circular DNA molecule to analyze that is 3,000bp (3 Kb) long. You...
You are given a circular DNA molecule to analyze that is 3,000bp
(3 Kb) long. You proceed to treat the DNA molecule with different
cutting enzymes and then you run the individual reactions on a gel.
The following gel is produced with each column representing a
different reaction (lane). In lane 1 a molecule weight ladder is
run. In lane 2, the circular DNA is NOT treated with any enzyme. In
lane 3 the DNA is treated with an enzyme...
A key difference between the nucleotides in DNA and those in RNA is that A. DNA...
A key difference between the nucleotides in DNA and those in RNA is that A. DNA has thymine, but RNA has uracil B. Both b and c are correct C. Both a and b are correct D. DNA has deoxyribose, but RNA has ribose E. DNA has phosphate but RNA does not
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There are some genomes that have been reported to be positively coiled instead of negatively supercoiled,...
There are some genomes that have been reported to be positively coiled instead of negatively supercoiled, which is the status of most genomes that we have studied. The genomes that are positively supercoiled seem to belong to viruses and cells that exist at very high temperatures. Why might positive supercoiling be an advantage at high temperatures? (Select all that apply.) 1) Positive supercoiling would allow the DNA to readily separate for transcription and replication. 2) At high temperatures, the condition...
Which of the following is a major difference between prokaryotes and eukaryotes? a. Eukaryotes have linear...
Which of the following is a major difference between prokaryotes and eukaryotes? a. Eukaryotes have linear chromosomal DNA, prokaryotes do not b. Eukaryotes live in extreme environments, prokaryotes do not c. Prokaryotes live in extreme environments, eukaryotes do not d. Only prokaryotes have histones, eukaryotes do no
Q9:The chromosomes of some viruses and bacteria are ______ and often ______. A Option A: linear;...
Q9:The chromosomes of some viruses and bacteria are ______ and often ______. A Option A: linear; single stranded B Option B: None of the above C Option C: linear; supercoiled D Option D: circular; single stranded E Option E: circular; supercoiled Q 5:In A-DNA A Option A: the glycosidic bond conformation and sugar pucker is dependent on the base. B Option B: the glycosidic bond is in the anti conformation with a C2'-endo sugar pucker. C Option C: the glycosidic...
This figure represents supercoiled, circular, double-stranded DNA: If this molecule of DNA originated as a linear molecule with a linking number (L) of 30, which was then circularized and unwound, the L for the unwound circular molecule would be O32 O 18 O 28 O 30 20
in regards to lane 3 of this gel, which band is nicked, linear
and supercoiled?
Lysis & Boiling Method Preps (Students) 45 ked ectrophoresis ests) wolts a nicked ) linear supercoiled 1% Agarose Gel Graduate Students Alkaline Lysis-Boiling Method Preps EcoRI-BamH-I Digests 02-14-2020 A2188
You are given a circular DNA molecule to analyze that is 3,000bp
(3 Kb) long. You proceed to treat the DNA molecule with different
cutting enzymes and then you run the individual reactions on a gel.
The following gel is produced with each column representing a
different reaction (lane). In lane 1 a molecule weight ladder is
run. In lane 2, the circular DNA is NOT treated with any enzyme. In
lane 3 the DNA is treated with an enzyme...
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