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You prepared a translational fusion of protein Z and the yellow fluorescent protein YFP (ZYFP) then...

You prepared a translational fusion of protein Z and the yellow fluorescent protein YFP (ZYFP) then expressed it in an animal cell line. You hypothesize that protein Z is a component of two different complexes in a cell. To test this idea, you intend to image Z-YFP using a fluorescence microscope equipped with a 60x objective (NA=0.9), and a 540 nm filter to detect YFP emission. Can you distinguish these complexes if they are approximately 330 nm apart? Explain why or why not, and what recommendations you would make if any are needed

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