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Starting in the nucleus, where the insulin gene is located, trace the path of insulin production...

Starting in the nucleus, where the insulin gene is located, trace the path of insulin production and secretion from the cell including the different organelles involved and each of their functions.
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Insulin production occurs in beta cells of islets of Langerhans in pancreas. The insulin gene is located on chromosome 11 of human beings. It transcribes to produce preproinsulin mRNA, which is regulated by transcription factor Pdx-1 prominently. Once the preproinsulin mRNA is transcribed in the nucleus, it enters endoplasmic reticulum (RER) for translation and forms preproinsulin. After the removal of signal peptide from preproinsulin (that guides its entry into SER), the proinsulin is subjected to specific endopeptidases to yield mature insulin. From SER the mature insulin is transported to golgi, where it is packaged into vesicles (called the secretory granules) and released into the cytoplasm. The secretory granules remain in the cytoplasm till there is signal for insulin release (increased glucose concentration in the blood). The secretion of insulin occurs through exocytosis, i.e., the secretory granules fuse with the plasma membrane and release the insulin in the islet blood supply.

So the course of production and secretion of insulin is: Nucleus--->Endoplasmic Reticulum--->Golgi--->Cytoplasm--->Blood

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