You have three solutions each containing the same concentration of a different protein. Two of these...
You have three solutions each containing the same concentration of a different protein.
Two of these proteins have 2 Trp in their sequence, whereas the third one only contains
one Tyr and one Phe. Which protein solutions will show the highest absorbance at
280nm? Why?
Homework Answers
Answer-
On comparison at a molar level, Tryptophan(Trp) molecule absorbs more light at 280nm, than either of Tyrosine(Tyr) and Phenylalanine(Phe) molecules. The first protein thus, shows the highest absorbance values at 280 nm, due to the presence of two Trp moieties. The second protein bears a Tyr and a Phe moeity, thereby, showing lesser absorbance than the former protein.
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