You have fused a mouse cell and a human cell and then treated the cell with specific antibodies that are covalently linked to fluorescent dyes (antibodies to mouse proteins - green; antibodies to human proteins - red). What does the cell look like immediately after fusion?
a) the cell is half red and half green
b) the and green labels are uniformly distributed across the entire membrane
c) the red and green labels are distributed in intermingled patched
d) the cell appears to be yellow in color
e) the cell appears to be brown in color
I think c is correct answer because chimera will produce in which some cell which have taken green will appear green and some taken red so appears red in patches.
You have fused a mouse cell and a human cell and then treated the cell with...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity chromatography but also to ___________. A. overexpress the proteins in bacteria B. to reduce toxicity of the foreign proteins C. for proper folding and keeping the proteins soluble D. to trace the cytological location of the foreign proteins 15. You have constructed an insulin-GST fusion protein and expressed it in E. coli. You want to separate the recombinant...
3. Suppose after adding HCl to ppt 16, you do not have any black solid. What, if anything, should you do to test for Co2 and Ni*? Qual Scheme: Group III: Co?(pink), N1" (green), Fet+ (yellow), Cr" (blue-grey), Mn(pink), AP (colorless), Zn? (colorless). Unknown B Solution: Use 10 dps of unknown sample and 10 dps of H20 and go to Procedure for Group III. Procedure for Group II: Add 2 dps NHCl soln, add 6M NHOH until just basic to...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
1) The Innocence Project uses DNA fingerprint testing to explore
the potential innocence of selected people convicted of crimes. The
Innocence Project discovered that the main reason that people are
wrongly convicted is because of
Select one:
a) trial judicial procedural errors.
b) police and legal system corruption.
c) mistakes made by the defense legal team.
d) mistakes made in forensic DNA testing.
e) incorrect eyewitness identification.
2) Gene expression in eukaryotic cells requires a change from a
highly condensed...
please help and thank you
Materials Needed per class: 1 box of 24 microscope slides of meiosis 1 1 box of 24 microscope slides of meiosis 2 red and yellow popbead chromosome kits Objectives To become familiar with the process of meiosis and to be able to identify the principal phases of meiosis To understand how the process of meiosis is similar to mitosis and how it differs from mitosis Introduction The genetic information of a cell is encoded in...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
Pretend you are the first response team for the following disaster: You are in a rural town. The tornado siren has gone off. A tornado has gone through the town. Many power lines are down, trees are uprooted and broken in half, and roads are totally blocked throughout the town by debris. Police are working on setting up traffic diversions. You see minor and major damage to homes and business, crumbling brick, missing roofs, collapsed walls, and a fire hydrant is gushing water...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing and understanding what is being done. I have copied and pasted some of the text from the article. Thank you! Abstract : Obesity, high-fat diets, and subsequent type 2 diabetes (T2DM) are associated with cognitive impairment. Moreover, T2DM increases the risk of Alzheimer's disease (AD) and leads to abnormal elevation of brain beta-amyloid levels, one of the hallmarks of AD. The psychoactive alkaloid caffeine...