Wild-type bacteria can grow on minimal medium. Four mutants that cannot grow on minimal medium but can grow on minimal medium supplemented with the nutrient "H" are isolated. It is suspected that metabolites T, P, and A are in the biochemical pathway for synthesis of H, so each mutant is tested for the ability to grow on minimal medium supplemented with these metabolites:
| A. | Mutant 1: can grow on minimal medium supplemented with T, but not P or A |
| B. | Mutant 2: is unable to grow on minimal medium supplemented with T, P, or A |
| C. | Mutant 3: is able to grow on minimal medium supplemented with A or T, but not P |
| D. | Mutant 4: can grow on minimal medium supplemented with T, P, or A. |
Which of the following is NOT consistent with this information?
| Mutant 3 blocks the conversion of metabolite P → metabolite A |
| Mutant 1 will cause a build up of metabolite A |
| Mutant 1 blocks the conversion of metabolite T → metabolite H |
| Mutant 2 blocks a step right before metabolite H |
| Mutant 4 blocks a step before all the metabolites |
***please explain your reasoning***
Wild-type bacteria can grow on minimal medium. Four mutants that cannot grow on minimal medium but...
Wild-type bacteria can grow on minimal medium. Four mutants that cannot grow on minimal medium but can grow on minimal medium supplemented with the nutrient “C” are isolated. It is suspected that metabolites A, B, and D are in the biochemical pathway for synthesis of C, so each mutant is tested for the ability to grow on minimal medium supplemented with these metabolites: Mutant 1: can grow on minimal medium supplemented with A, but not B or D Mutant 2:...
Four nutritional mutants in Neurospora will grow if arginine is added to minimal medium. Some of these mutants also will grow if other substrates chemically related to arginine are added to minimal medium. The growth patterns are show in the table (+ = growth and 0 = no growth). A. What is the sequence in the metabolic pathway of arginine (order of metabolites)? B. Indicate where each mutant is blocked in the pathway? Supplements (metabolites) added to minimal medium...
2. Four auxotrophic mutants in Neurospora crassa.(common bread mold) will grow if arginine is added to minimal medium. Some of these mutants will also grow if other chemicals in the arginine anabolic pathway are added to minimal medium. The growth pattems are show in the table (+ = growth and 0 - no growth) Metabolites added to minimal medium Glutamic Semialdehyde Arginine Mutants Precursor Citrulling . Omithing A. What is the order of metabolites in the pathway? B. Indicate with...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
Please answer 54, 55, 61,62,64,66,70,72,82,83
on the facs that newly replicated DNA strands are ethylated relative to older DNA 54, Ir used u ished to study the SOS response in Escherichi col, which of the following esperimental procodures could b A.All ofthe choices are correct Expoe cells to nonionising radiation for 20 es C. Espose cells to soniring radiation for 10 minutes Transfer log phase cells frees complete medium to thymine minimal medium What wouild be the hypothetical outcome if...
Title: Development of an Adhesion Assay and Characterization of an Adhesion-Deficient Mutant of Pseudomonas fluorescens RESULTS Bacterial adhesion to sand columns. In the initial column assays with soil-and-sand mixtures, P. fluorescens PfO-1 and E. coli SLH25 had the highest and lowest adhesion values, respectively. Therefore, the sand column assay was optimized to yield the greatest difference in percent adhesion for these two strains. PfO-1 bacteria grown in minimal medium to the logarithmic phase were washed and suspended in either minimal...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
explaim the mechanisms amd toxological effects if type 1
diabetes in this article
Exposure to arsenic in drinking water is associated with increased prevalence of diabetes. We previously reported an association of diabetes and urinary concentration of dimethylarsinite (DMAS"), a toxic product of arsenic methylation by arsenic (+ 3 oxidation state) methyltransferase (AS3MT). Here we examine associations between AS3MT polymorphism, arsenic metabolism and diabetes. Fasting blood glucose, oral glucose tolerance and self-reported diagnoses were used to identify diabetic individuals. Inorganic...