if the plasmid concentration is 100 ng/ul how much ul need to be added to the 20 ul reaction?
What if the concentration is 10 ng/ul how much ul would need to be add to the 20 ul reaction?
if the plasmid concentration is 100 ng/ul how much ul need to be added to the...
The starting concentration of the plasmid DNA is 100 ngul and you added 2μ1 to your cells. The cells were diluted to one ml prior to plating. First, determine how many transformants you would have if you plated the entire 1 ml of cell:s. Remember, you only plated half or your transformation reaction on plates 1 and 2. Add the number of transformants on plates 1 and 2, and then multiply by 2 for the total number transformants. Record that...
1.The plasmid purified is a low copy number plasmid and the results indicate there is much more DNA present in the sample than expected. Why might this be the case? What step in the extraction process might be the cause? 2. The perfect DNA sample is extracted at a concentration of 95 ng/ul. How many nanograms are present in 200 ul of DNA extract? 3. Explain how to create a working stock of 50 ng/ul from the 95 ng/ul DNA...
How would you make 600 uL of DNA at a concentration of 60 ng/ul from stock DNA that has a concentration of 300 ng/ul and TE buffer?
5 ul (10ng.ul) of ptud plasmid control, 2 colonies grown 100ng of DNA was used for ligation, 25ng DNA was used for transformation, 1ml was the total volume of cells grown, 100 ul was plated, 130 colonies were grown 1. What was your transformation efficiency for control DNA pTUD at concentration of 10 ng/ul)? 2. What was transformation efficiency for experimental ligation (you will need to calculate approximate concentration from the steps of the cloning procedure you performed)?
You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing your plasmid (concentration = 500 ng/μl) with EcoRI, which is provided in a standard 50% glycerol solution at 10 units/μl. Your enzyme also comes with an appropriate 10X reaction buffer. Taking into account the final allowable glycerol concentration, you want to add the maximum amount of EcoRI, to achieve complete digestion of 1 μg plasmid DNA in a total volume of 20 μl. Fill...
Based on concentration from Nanodrop, determine volume of RNA in µL needed for 5 µg. For example if concentration was 77.4 ng/µL, then: (5 µg)(1000 ng/µL)(x µL / 91.6 ng) = _____ µL Concentration from Nanodrop = 91.6 ng/µL Since DNase I Reaction buffer is 10X and volume for reaction is 100 µL, how much 10X DNase I reaction buffer will you to need to obtain final concentration of 1X? Based on volume RNA and 10X DNase I reaction buffer...
For the PCR reaction you have to dilute the cDNA to 100 ng/µL. Your cDNA has a concentration of 700 ng/µL. Taking 10 µL of this cDNA, by what factor to you have to dilute it and how much PCR water do you add?
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock concentration of Linker B-1 μg/ul in TE buffer The concentration for each linker needed for the experiment is 20 ng/ul. How would you get this concentration?
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock concentration of Linker B-1 μg/ul in TE buffer The concentration for each linker needed for the experiment is 20 ng/ul. How would you get this concentration?
When setting up the polymerase chain reaction, approximately 200 ng of DNA should be added in a 50 ul reaction. How many ul of the above (it was 115 ng/ul) undiluted DNA solution should be added to the PCR tube
qRT-PCR The reaction mixture for qRT-PCR requires the following: Taq Mix : 1 uL, SYBR Green Mix: 25 uL, Forward primer : 1 uL, Reverse primer : 1 uL, ROX Reference Dye : 1 uL, Template : 50 ng RNA , Volume up to 50 uL with nuclease free water. If you are going to make a 20 uL reaction mixture and have a 10 ng/uL RNA sample, how much of each of the components do you need? You also...