HomeworkLib
Question

The starting concentration of the plasmid DNA is 100 ngul and you added 2μ1 to your cells. The cells were diluted to one ml prior to plating. First, determine how many transformants you would have if you plated the entire 1 ml of cell:s. Remember, you only plated half or your transformation reaction on plates 1 and 2. Add the number of transformants on plates 1 and 2, and then multiply by 2 for the total number transformants. Record that number here. You recorded this above on the previous page. 1,008 Second, convert ng of plasmid added to the transformation to μg. Record that number below and show your work. Finally, do a little division to calculate number of transformants obtained per μg of plasmid. Report your efficiency as # of transformants per ug (or TFsug). Show your work and dont forget your units.
science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

you havent provide information about cells.

if the transformation is successful and you plated all the culture then you'll get a lawn of cells. and if it is unsuccessful you'll get nothing on the plate.

1000 ng = 1ug.

conc = 100ng/ul. you added 2ul means 200ng of DNA means 0.2 ug of DNA.

0 0
Add a comment
Know the answer?
Add Answer to:
The starting concentration of the plasmid DNA is 100 ngul and you added 2μ1 to your...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated lab...

    Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...

  • You add (1.04x10^0) ng of plasmid DNA in 50 µL to 100 µL of competent E....

    You add (1.04x10^0) ng of plasmid DNA in 50 µL to 100 µL of competent E. coli cells and incubate the mixture on ice for 20 min. Following a heat-shock treatment, the cells are incubated at 37 C for 30 min. At the end of the incubation you spread (1.2000x10^2) µL of the transformation mix on an agar plate and incubate the plate overnight at 37 C. The next morning you observe (6.8400x10^2) colonies on the plate, each colony having...

  • During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of...

    During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of 0.08 ug ul. You transformed 191 UL of competent E. coli with 100L of PGLO DNA. You added 191 UL of LB broth to the transformation before plating 100 uL of the culture to a plate labeled +POLO, LB, Amp, Ar. After growing the plates overnight, 136 fluorescent colonies (transformants) were counted. What was the average transformation efficiency of your work?

  • 5 ul (10ng.ul) of ptud plasmid control, 2 colonies grown 100ng of DNA was used for...

    5 ul (10ng.ul) of ptud plasmid control, 2 colonies grown 100ng of DNA was used for ligation, 25ng DNA was used for transformation, 1ml was the total volume of cells grown, 100 ul was plated, 130 colonies were grown 1. What was your transformation efficiency for control DNA pTUD at concentration of 10 ng/ul)? 2. What was transformation efficiency for experimental ligation (you will need to calculate approximate concentration from the steps of the cloning procedure you performed)?

  • PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone,...

    PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone, 0.5% yeast extract, 10 mM NaCL (F.W. 58), 2.5 mM KCl (F.W. 74.5), 10 mM MgSO4 (F.W. 246.5), 10 mM MgCl2 (F.W. 203.3), 20 mM glucose (F.W. 180.16). Determine the amounts of each to make 500 mL of SOC. 2) Determine the number of moles in 0.05 μg of 300 bp insert DNA. There are 660 g/mol-bp. What is the molar ration of insert...

  • You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing...

    You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing your plasmid (concentration = 500 ng/μl) with EcoRI, which is provided in a standard 50% glycerol solution at 10 units/μl. Your enzyme also comes with an appropriate 10X reaction buffer.  Taking into account the final allowable glycerol concentration, you want to add the maximum amount of EcoRI, to achieve complete digestion of 1 μg plasmid DNA in a total volume of 20 μl. Fill...

  • Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme...

    Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme Eco R1. A map of the plasmid is shown here. The entire plasmid is 6000 bp, and there are Eco R1 restriction sites at 1500 bp, 2000 bp, and 4000 bp. You’re going to run the entire volume of the digest on a gel, and you want to cut just enough DNA to have 50 ng in the smallest band on your gel. Starting...

  • 1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...

    1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation occurred on which agar plate (s)? What evidence do you have that the bacteria were transformed here? 3. Which plates have glowing growth? Explain what causes bacteria to glow. II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...

  • Need all answers with explanations please 1. (2 pts) In your own words, briefly but completely...

    Need all answers with explanations please 1. (2 pts) In your own words, briefly but completely differentiate antiseptics and disinfectants from each other. Do not just write down their definitions; highlight how they are different 5. (2 pts) Ethan and Ela performed the same handwashing as done in lab session 7, and plated basin C as your group plated the assigned basin. For plates with 0.4 mL, they counted 650 and 560 CFU. Their 0.2 mL plates had 400 and...

  • Please answer both! I would appreciate it! Show all your work as well. I’m having trouble...

    Please answer both! I would appreciate it! Show all your work as well. I’m having trouble with dilutions. Thank you! You have 99 mL and 9 mL dilution blanks available to you. Draw and label a protocol by which you could make three plates that contained a 1 X 10, 1 X 104 and 1 X、105 dilution. 2. 3. A culture was diluted by adding a 0.1 mL aliquot to 0.9 mL water. Then, 0.1 mL of this dilution was...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT