Yersinia enterocolitica is motile at 22-290C and is non motile at 370C. It is gram negative, non lactose fermenting, glucose and sucrose fermenting non H2S producing bacteria. So in MacConkey agar it will from white colonies and in triple sugar iron slants it will have have yellow slant and yellow agar butt indicative of glucose and sucrose fermentation.
What is the characteristic motility of Yersinia enterocolitica? How does sugar fermentation of this organism correlate...
How does glucose fermentation correlate with oxygen requirement?
Micro Lab: Enterbacteriacease
Lab
7; Enterobacteriacease Question TSI/SIM
Yersinia pestis ! Name the sugars in a TSI slant. 20. 21. TSI slants are incubated with the caps loose/tight. 22. Most bacteria will grow best at 35 degrees Centigrade. There are two exceptions. Yersinia will grow at while Campylobacter is incubated at __degrees centigrade. 23. Name an organism that gives each TSI result. A/A gas-- K/A-- K/A H2S+-- K/N- what three tests can be determined from SIM media? List an organism...
4) In the absence of O2 fermentation will occur, why?.....what does it yield that the organism needs from these reactions? Why? 5) Gluconeogenesis is the production of glucose from non-carbohydrate precursors. a. What distinguishes glycolysis from gluconeogenesis? (hints: substrates, energetics of reactions, where do they occur) b. What molecule is a key regulator of glycolysis and gluconeogenesis? What does it do? (p. 245)
1. Why do yeasts perform alcohol fermentation? 2. What is the chemical reaction for fermentation? 3. Which are the reactants in this reaction? Which are the products? 4. What is the gas that accumulates in the bag, causing it to inflate? In addition to this gas, what is another product of this process? Can you think of one example when either of these products is useful during cooking/baking? 5. How does inflation of the bag correlate with the amount of...
TSI Triple Sugar Iron Agar Data Sheet The enterics, specifically coliforms, will show what result in the TSI? What is reversion and what organisms showed reversion in the TSI? What does a black color in the butt of the slant indicate in the TSI? What is the pH indicator in the TSI and what is the purpose of the pH indicator? Do all organisms use the sugars in the TSI? What organisms do not utilize the sugars and what is...
1. what is the seven practices of biosafety? 2.how to aseptically inoculate a culture, and streak a plate for isolation. 3. Know the names of the following differential tests, the components of these tests (dyes, indicators, sugars, etc), what organisms they test for, what biochemical reactions they test for, and be able to interpret a result. Phenol red broth (5-2) Catalase test (5-5) Nitrate reduction test (5-7) Citrate test (5-8) Decarboxylation test (5-10) Urease test (5-14) Sulfur, Indole, Motility (SIM)...
1. How does true motility differ from Brownian movement? 2. What could happen if you would touch the bottom of the motility agar with your needle during inoculation? 3 Explain why the following steps are essential during subculturing: a) Flaming the loop or needle prior to and after each inoculation. b) Cooling the loop or needle prior to obtaining the microorganism c) Holding the test tube caps in the hand as opposed placing them on the bench top. d) Flaming...
Questions 1. What does the term IMVIC mean? 2. Why is the IMVIC useful in identifying Enterobacteriaceae? Are further biochemical tests necessary for complete identification? 3. What diagnostic test differentiates Proteus and Providencia species from other Enterobacteriaceae? om other Enterobacteriaceae 4. How is E. coli distinguished from P. vulgaris on MacConkey agar? On a TSI slant? 5. Instead of TSI, why would a slant medium containing only dextrose and lactose (not sucrose) be preferable for detecting Y enterocolitica? Name such...
In a cell, why must NADH be reoxidized? How does this happen in an organism that uses respiration? Fermentation? 2. Explain the chemiosmotic mechanism of ATP generation. How does oxidative phosphorylation compare with substrate level phosphorylation found in glycolysis and the Kreb’s cycle? 3. Which of the following yields the greatest energy for a cell: fermentation, anaerobic respiration, or aerobic respiration? Which yields the least? Why? 4. Explain what happens to glucose during glycolysis and respiration in terms of oxidation...
In a cell, why must NADH be reoxidized? How does this happen in an organism that uses respiration? Fermentation? 2. Explain the chemiosmotic mechanism of ATP generation. How does oxidative phosphorylation compare with substrate level phosphorylation found in glycolysis and the Kreb’s cycle? 3. Which of the following yields the greatest energy for a cell: fermentation, anaerobic respiration, or aerobic respiration? Which yields the least? Why? 4. Explain what happens to glucose during glycolysis and respiration in terms of oxidation...