Could a plaque assay be used to detect a change in viral quantities like TCID50 assay...
Could a plaque assay be used to detect a change in viral quantities like TCID50 assay can. Why or why not?
Homework Answers
No plaque assay couldn't be used to detect change in viral quantities like TCID50 assay could because in this TCID50 dose of virus must be determined then also if the virus does not form plaques . Plaque formation is important in plaque assay . And plaque assay used a time interval of 14 days for quantification of virus .
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Could a plaque assay be used to detect a change in viral
quantities like TCID50 assay...
You repeat the viral plaque assay adding 10 microliters of viral solution to 990 microliters of...
You repeat the viral plaque assay adding 10 microliters of viral solution to 990 microliters of LB. You perform this serial dilution five times. You add 10 microliters from each dilution to the corresponding host bacteria molten top agar mixture which is then spread onto an LB agar plate. The next day you count 39 plaques on the fourth plate. Why is it that on plate 3 the number of plaques will be 39 x 10^2 and on plate 5...
In plaque assay, why is soft agar used in the top layer? Why is it important...
In plaque assay, why is soft agar used in the top layer? Why is it important that bacterial cells should be in the exponential phase for this experiment?
In Chapter 21, we performed the plaque assay in which the T4 bacteriophage was used to...
In Chapter 21, we performed the plaque assay in which the T4 bacteriophage was used to infect E. coli strain B cells. Select one of the scenarios below, indicate the expected result, and describe what could have caused the observed result. You are asked to repeat the plaque assay using the T4 bacteriophage with E. coli strain B. After a 24 hour incubation period at 37°C, you observe no plaques on all of your plates. You are asked to repeat...
which colorimetric test could be used to assay a person's urnie and what color change could...
which colorimetric test could be used to assay a person's urnie and what color change could indicated mild diabetes?
You are provided with culture fluid from virus infected cells and you would like to know the infectious viral titer. Exp...
You are provided with culture fluid from virus infected cells and you would like to know the infectious viral titer. Explain the theory and methodology of how you would determine the infectious titer of the sample using a plaque assay. [ESSAY TYPE QUESTION]
pls help, ty ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
4. Bradford assay. In your prac you used the Bradford assay to determine the protein concentration...
4. Bradford assay. In your prac you used the Bradford assay to determine the protein concentration (1.5 marks) a) Explain at a molecular level how the color development of the assay works. Answer must be less than 100 words. (0.5 marks) b) Explain why you can use Bovine Serum Albumin as standard protein to determine the concentration of milk proteins. Answer must be less than 50 words. (0.5 marks) c) Why is it important to use diluted milk fraction samples...
What physical properties of an explosive compound could be used to detect a bomb before it...
What physical properties of an explosive compound could be used to detect a bomb before it detonates?
3. Why can we assume in the Biuret assay that BSA (used in the standards) and...
3. Why can we assume in the Biuret assay that BSA (used in the standards) and casein (our protein of unknown concentration) give about the same amount of A550 per mg? 4. To calibrate the Ninhydrin assay, glycine was used. Draw the structures of Glycine, Arginine, and Threonine. Instead of glycine, would it be acceptable to use Arginine or Threonine? Explain!
a DNA microarray (also called a DNA chip) can be used to____ A) detect RFLPs B)...
a DNA microarray (also called a DNA chip) can be used to____ A) detect RFLPs B) generate mutations in a gene of interest C) detect mutations that may indicate a risk of disease D) determine protein translation levels If you could explain the reasoning it would really help, thanks!
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
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