Why do you bacterial cells exposed to visible light following a significant UV radiation have a...
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Mutation using ultraviolet (UV) light is an example of Non-ionizing radiation. Ultraviolet (UV) light excites the electrons in molecules which damages the DNA by dimerization of thymine residues. The dimer changes the shape of the DNA that cause problems during replication. One mechanism for repairing thymine dimers is the DNA photolyase mediated enzymatic photoreactivation. This enzyme uses energy from visible light to cleave the dimer and repair DNA. Thus cells that are exposed to visible light following a significant UV radiation have a greater survival rate (due to FNA repair) than cells kept in the dark.
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Why
do you bacterial cells exposed to visible light following a
significant UV radiation have a...
and Lab Exer n this experiment you will evaluate effect of UV light on bacterial growth. UV light affects growth by interfering with DNA, resulting in thymine dimer formation. The dimerization...
and Lab Exer n this experiment you will evaluate effect of UV light on bacterial growth. UV light affects growth by interfering with DNA, resulting in thymine dimer formation. The dimerization is confined within adjacent thymine residues on same strand (intrastrand ). These dimmer lesions have consequence on DNA functions including interference in DNA replication, transcription etc, so UV exposure of cells can lead to lethal effects for bacterial multiplications. This forms the basis for UV method to control bacterial...
Results A. Effect of UV Radiation Record the results for each plate at follows (see images...
Results A. Effect of UV Radiation Record the results for each plate at follows (see images below): (-) no growth 1+ light growth 2+ medium growth 3+ heavy growth Also, note any differences in appearance of growth on cach of the plates. 15 30 60 ates) Bacteria Lid off UV light exposure time Lid off Lid off Lid off 15 sec 30 sec 60 sec O sec Lid on 60 sec (control) Serratia marcescens Based on these results, answer the...
Which culture should be able to survive the longest exposure to UV (SA or BS)? Why?...
Which culture should be able to survive the longest exposure to UV (SA or BS)? Why? 7. Do you expect to see a difference between the growth on the side of the plate that was under the index card and the side that was open? If so, explain what you expect to see. 8. Some cells have a means of protecting themselves against radiation. Can you name a few structures that might help protect a cell from UV light?
Lab Exercise - Ultraviolet Light-Lethal Effects Please review the below videos as well as read introduction,...
Lab Exercise - Ultraviolet Light-Lethal Effects Please review the below videos as well as read introduction, materials, methods & procedures in the lab manuals and answer the following questions. https://www.youtube.com/watch?v=z4arnMlhbpE&app=desktop https://www.youtube.com/watch?v=OMN9ZGSHZW 1) Why was half of each plate covered with an index card? 2) Suppose you've tested the effect of UV radiation on bacterial growth for E. coli vs. S. aureus plates. How did the growth on E. Coli plates differ from the growth on the S. aureus plates that...
Electromagnetic Waves UV Radiation Some of us are all too familiar with the ill- effects of...
Electromagnetic Waves UV Radiation Some of us are all too familiar with the ill- effects of over-exposure to ultraviolet (UV) electromagnetic radiation, namely, sunburns (solar erythema). UV radiation can kill the skin cells immediately below the surface (part of the epidermal layer). Over the course of several minutes or several hours, as enough skin cells are killed or damaged, the body's natural immune response is triggered. The body responds to the damage with increased blood flow to the capillary bed of...
Why do the phycobilins found in red algae trap more light energy than chlorophyll in deep...
Why do the phycobilins found in red algae trap more light energy than chlorophyll in deep water? Which of the green algae are motile? How do these organisms move? How are the pseudopodia of these amoebas different than the pseudopodia of Foramens and Radiolarians? Can you see any internal structures in the bacteria? Why or why not? Given the differences between bacterial and animal cells as previously discussesed, what aspects of abcterial structure might be good targets for antimicrobial agents?...
30. Which of the following is not true about the electromagnetic spectrum? a. ultraviolent light (UV)...
30. Which of the following is not true about the electromagnetic spectrum? a. ultraviolent light (UV) has a shorter wavelength than does visible light b. the energy of a wave is continuous c. the energy of a wave is proportional to the frequency d. The energy of a wave in inversely proportional to the wavelength True/false 31 The shape of a molecule influences the amount of radiation that can be absorbed 32_ __Carbon atom always forms four bonds 33___Water vapor...
4. Development of organelles Aa Aa 3 Modern eukaryotic cells are very complex and have many...
4. Development of organelles Aa Aa 3 Modern eukaryotic cells are very complex and have many features that allow them to function efficiently. These complexities took a very, very long time to evolve and likely progressed as a series of small improvements that were naturally selected. For example, modern eukaryotic cells have internal membranes surrounding their organelles and the nucleus. This arrangement likely arose when the cellular membranes of some ancient bacterial cells accidentally folded to become internal membranes. Which...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
Pu010 2. You are studying a bacterial protein, Pmp 7, that pumps one potassium (Kt) ion...
Pu010 2. You are studying a bacterial protein, Pmp 7, that pumps one potassium (Kt) ion into the cell for each calcium ion (Ca²+) that is moved out of the cell. Pmp 7 uses energy from light to pump these ions across the membrane. Experiment 1: You make an artificial membrane vesicle that has Pmp7 as the only protein on the membrane. Pmp 7 is orientated on this artificial membrane vesicle as it is oriented in a bacterial cell, and...
and Lab Exer n this experiment you will evaluate effect of UV light on bacterial growth. UV light affects growth by interfering with DNA, resulting in thymine dimer formation. The dimerization is confined within adjacent thymine residues on same strand (intrastrand ). These dimmer lesions have consequence on DNA functions including interference in DNA replication, transcription etc, so UV exposure of cells can lead to lethal effects for bacterial multiplications. This forms the basis for UV method to control bacterial...
Results A. Effect of UV Radiation Record the results for each plate at follows (see images below): (-) no growth 1+ light growth 2+ medium growth 3+ heavy growth Also, note any differences in appearance of growth on cach of the plates. 15 30 60 ates) Bacteria Lid off UV light exposure time Lid off Lid off Lid off 15 sec 30 sec 60 sec O sec Lid on 60 sec (control) Serratia marcescens Based on these results, answer the...
Lab Exercise - Ultraviolet Light-Lethal Effects Please review the below videos as well as read introduction, materials, methods & procedures in the lab manuals and answer the following questions. https://www.youtube.com/watch?v=z4arnMlhbpE&app=desktop https://www.youtube.com/watch?v=OMN9ZGSHZW 1) Why was half of each plate covered with an index card? 2) Suppose you've tested the effect of UV radiation on bacterial growth for E. coli vs. S. aureus plates. How did the growth on E. Coli plates differ from the growth on the S. aureus plates that...
30. Which of the following is not true about the electromagnetic spectrum? a. ultraviolent light (UV) has a shorter wavelength than does visible light b. the energy of a wave is continuous c. the energy of a wave is proportional to the frequency d. The energy of a wave in inversely proportional to the wavelength True/false 31 The shape of a molecule influences the amount of radiation that can be absorbed 32_ __Carbon atom always forms four bonds 33___Water vapor...
4. Development of organelles Aa Aa 3 Modern eukaryotic cells are very complex and have many features that allow them to function efficiently. These complexities took a very, very long time to evolve and likely progressed as a series of small improvements that were naturally selected. For example, modern eukaryotic cells have internal membranes surrounding their organelles and the nucleus. This arrangement likely arose when the cellular membranes of some ancient bacterial cells accidentally folded to become internal membranes. Which...
Pu010 2. You are studying a bacterial protein, Pmp 7, that pumps one potassium (Kt) ion into the cell for each calcium ion (Ca²+) that is moved out of the cell. Pmp 7 uses energy from light to pump these ions across the membrane. Experiment 1: You make an artificial membrane vesicle that has Pmp7 as the only protein on the membrane. Pmp 7 is orientated on this artificial membrane vesicle as it is oriented in a bacterial cell, and...
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