Question

•The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm3mol-1cm-1 and 1.12 x 104 dm3mol-1cm-1 , respectively, and at 280 nm they are 5.40 x 103 dm3mol-1cm-1 and 1.50 x 103 dm3mol-1cm-1 . The absorbance of a sample obtained by hydrolysis in a 1 cm cell was 0.660 at 240 nm and 0.221 at 280 nm. What are the concentration of the two amino acids?

•Without calculating, which amino acid is more abundant?

•Calculate the concentrations