The next day after transforming bacterial cells with the products of a ligation reaction, you discover...
The next day after transforming bacterial cells with the products of a ligation reaction, you discover that the plate used to select for transformants has no colonies at all. Give two reasons for this unfortunate result. Write two sentences or less for each reason.
Homework Answers
1. The ligation molecule should maintain an appropriate concentration . If the concentration is high ligation will not be carried out. Thus no colonies will be formed.
2. The cells will not get transformed if the Dna is not ligated. Thus the host will not become competent and will not produce result against selective marker.
3. Colonies will not be formed if the cells are not viable and thereby no transformation will takes place.
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The next day after transforming bacterial cells with the
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