Question

Consider the degradation of glucose to pyruvate by the glycolytic pathway:

glucose+2ADP+2Pi+2NAD+→2pyruvate+2ATP+2H2O+2NADH+2H+

Part A

Calculate ΔG for this reaction at pH=7.4 and 37 ∘C when the reactants and products are at the concentrations given below.

ATP=4.00mM	Pi=5.90mM	ADP=220μM
glucose=5.10 mM	pyruvate=64.0 μM
NAD+=350μM	NADH=14.5μM	CO2=15.0torr

half reaction			E∘′(V)
NAD++H++2e−	⟶	NADH	−0.315
2pyruvate+6H++4e−	⟶	glucose	−0.590
pyruvate+NADH+2H+	⟶	ethanol+NAD++CO2	ΔG∘′=−64.4kJ/mol
ATP+H2O	⟶	ADP+Pi+H+	ΔG∘′=−32.2kJ/mol

Answer 1