Question

1- Consider the fermentation pathways of Clostridium as diagrammed. A common goal of metabolic engineering is to focus the metabolism of the cell to yield just one or two primary products rather than the entire spectrum of products. Which of the following overall outcomes (these are not balanced reactions) could be metabolically engineered while preserving a balanced redox state (NAD+/NADH ratio)? Write Yes or No in the blank. 2ADP - 2ATP + Hexose I 2 Pyruvate NAD NADH 2 CO2 Acety CoA +2 NADH ADP- ATP + NAD Acetate Acetoacetate HADH+H* NAD Ethanol Acetaldehyde Acetoacetylco -NADH 2NAD Butyry CoA -NADH+H* NAD Acetone + CO2 Butyryl phosphate Butyraldehyde ADP- NADPHH* NADP Butanol ATP Butyrate Glucose → 2Acetate + 4ATP 2Glucose → Butanol + Acetone +4ATP Glucose → Butyrate + 3ATP Glucose + 2Acetone + 2ATP Glucose → Butanol + 2ATP Pick one of the cases for which you answered “No” and explain in a few words why production of this product is not feasible.

Answer 1

A is Yes.

B is No.

C is No.

D is Yes.

E is No.

E is No because as we can see one glucose give rise to 2 pyruvates with gain of 2 ATP. Then 2 pyruvate will give rise to 2 Acetyl CoA and then These 2 Acetyl CoA will form 2 butanol So 2 butanol will form not one.