1. A clear area (free of growth) around an antibiotic disk on this Kirby Bauer plate...
1. A clear area (free of growth) around an antibiotic
disk on this Kirby Bauer plate indicates that . . .
A. The bacterium is susceptible to the drug in vitro (i.e. in lab
media).
B. The bacterium is resistant to the drug in vitro.
C. The bacterium uses the drug as a nutrient source.
D. The drug will be effective against the bacterium in vivo (in the human body)
E. The drug is not soluble in water/agar.
Homework Answers
Hello
Answer is A, The bacterium is susceptible to the drug in vitro (i.e lab media)
Kirby bauer test measure antibiotic sensitivity . A Clear area or zone of no growth in the immediate vicinity of disc indicates susceptibity to the antimicrobial or antibiotic inhibit bacteria microbial growth
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1. A clear area (free of growth) around an antibiotic
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can you please help me with Qs 1-6 thanks ! LAB EXERCISE #6: Kirby-Bauer Disk Diffusion...
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Background
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A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
The physician orders a lab technician to perform a Kirby Bauer disk diffusion assay and finds that the organism is resistant to the following antibiotics: 1) Penicillin, 2) Ampicillin, 3) Methicillin, and 4) Trimethoprim/Sulfamethoxazole. The physician asks the man about any drug allergies and other pre-disposing factors that could impact anti-microbial therapy. The man is allergic to all of the beta-lactam antibiotics, all cephalosporins, and does not tolerate sulfa drugs well. The man also has a form of hepatitis from...
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Background
Questions
Bacteria in the environment must be controlled to reduce the chance of spreading the bacteria and possible infectious disease. There are many methods of controlling bacterial numbers; heat, chemicals, drugs and radiation are a few. Each control method targets something within the cell either structurally or metabolically that leads to cellular death. Microbes are most vulnerable during the exponential growth phase, when there is a high level of metabolism and cellular structures are being created. Many control methods...
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